Characterization of fatty acid conversion enzymes of soybean and their application for improvement of soybean oil quality

大豆脂肪酸转化酶的表征及其在大豆油品质改良中的应用

• 批准号: 08456005
• 负责人: TAKAGI Yutaka
• 金额: $ 1.6万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456005/
• 关键词: soybean; fatty acid; desaturation enzymes; 脂肪酸転換酵素; 不飽和化酵素遺伝子; 脂肪酸組成; ステアリン酸; オレイン酸; リノレン酸

Previously, we have reported to establish many fatty acid-mutants from X-ray irradiated soybean progenies.In this research, we analyzed their mutants using genetical and molecular biological approach, and then identified the gene family that is involved in fatty acid synthesis pathway. These results lead to conclusion that sop] and sop2 genes control the high palmitic acid character, st1 and st2 genes control the high stearic acid character, ol gene controls high oleic acid character, and fan and fanx genes control low linolenic acid character, respectively.We could isolate some cDNA clones encoding stearoyl-ACP desaturase, microsomal omega-6 fatty acid desaturase and microsomal omega-3 fatty acid desaturase from soybean seed cDNA library. Three of them were identified as corresponding clones to stl, ol and fan genes, respectively.Furthermore, we analyzed the expression of these genes under phytohormone treatments, and found that mRNA accumulations of both plastidal and microsomal omega-6 desaturase genes coordinately decreased, in contrast, that of omega-3 desaturase genes coordinately increased.

此前，我们曾报道过从X射线照射的大豆后代中建立了许多脂肪酸突变体，本研究采用遗传学和分子生物学方法对其突变体进行分析，进而鉴定出参与脂肪酸合成途径的基因家族。结果表明，sop_1和sop_2基因控制高棕榈酸性状，st_1和st_2基因控制高硬脂酸性状，ol基因控制高油酸性状，fan和fanx基因控制低亚麻酸性状。来自大豆种子cDNA文库的微粒体ω-6脂肪酸去饱和酶和微粒体ω-3脂肪酸去饱和酶。进一步分析了这些基因在植物激素处理下的表达情况，发现细胞质和微粒体ω-6去饱和酶基因的mRNA积累量协同下降，而ω-3去饱和酶基因的mRNA积累量协同增加。

项目成果

Y.Yamashita, T.Kinoshita, S.M.Rahman, K.Okada, T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies : I.Low linolenic acid mutants with a microsomal omega-3 fatty

Y.Yamashita、T.Kinoshita、S.M.Rahman、K.Okada、T.Anai 和 Y.Takagi：“大豆突变体及其后代脂肪酸合成的限制性片段长度多态性的遗传分析：I.低亚麻酸突变体，具有

Y.Yamashita,T.Kinoshita,S.M.Rahman,K.Okada,T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies:I.Low linolenic acid mutants with a microsomal ω-3 fatty acid desa

Y.Yamashita、T.Kinoshita、S.M.Rahman、K.Okada、T.Anai 和 Y.Takagi：“大豆突变体及其后代脂肪酸合成的限制性片段长度多态性的遗传分析：I.低亚麻酸突变体微粒体 ω-3 脂肪酸 desa

T.Kinoshita, S.M.Rahman, T.Anai and Y.Takagi: "Inter-locus relationship between genes controlling palmitic acid contents in soybean" Breed.Sci.48. 377-381 (1998)

T.Kinoshita、S.M.Rahman、T.Anai 和 Y.Takagi：“控制大豆棕榈酸含量的基因之间的位点间关系”Breed.Sci.48。

S.M.Rahman, T.Kinoshita, T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies : III.High stearic acid mutants with a stearoyl-acyl carrier protein desaturase cDNA

S.M.Rahman、T.Kinoshita、T.Anai 和 Y.Takagi：“限制性片段长度多态性对大豆突变体及其后代脂肪酸合成的遗传分析：III.具有硬脂酰基载体蛋白去饱和酶 cDNA 的高硬脂酸突变体

S.M.Rahman, Y.Takagi and T.Kinoshita: "Genetic control of high oleic acid content in the seed oil of two soybean mutants" Crop Sci.36. 1125-1128 (1996)

S.M.Rahman、Y.Takagi 和 T.Kinoshita：“两种大豆突变体种子油中高油酸含量的遗传控制”Crop Sci.36。