A SEARCH OF MICROBES PATHOGENIC FOR DIFFICULT-TO-CONTROL INSECT PESTS

难以控制害虫的致病微生物的研究

• 批准号: 08456030
• 负责人: OHBA Michio
• 金额: $ 4.8万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456030/
• 关键词: Bacillus thurin-giensis; diamondback moth-toxicity; novel Cry protein; Antarctica; microsporidian; Nosema; Pleistophora; Brazil; 親規Crg蛋白質; Nesema; 鱗翅目毒性; Cry9D δ-内毒素; 双翅目毒性; Cry19B δ-内毒素; Nosema mesnili; Nosema bombysis

(1) Gene sequence analysis revealed that the Cry proteins of the five Lepidoptera-specific Bacillus thuringiensis strains, that belong to the three H serovars (fukuokaensis, sotto, and galleriae) and produce spherical parasporal inclusions, are of the single category, a novel class belonging to the Cry9 delta-endotoxin.(2) Of 1713 Japanese isolates of Bacillus thuringiensis, 609 (35.5%) exhibited high larvicidal activity against the diamondback moth, Plutella xylostella. Of these toxic isolates, 605 belonged to the H serotypes 1-10. When five selected highly toxic isolates, belonging to four H serovars (sumiyoshiensis, 2 isolates ; fukuokaensis, 1 ; canadensis 1 ; morrisoni, 1), were examined for their delta-endotoxins, the four isolates, excluding the morrisoni isolate, were assigned to the Cry9D-producing strains.(3) Isolation experiments failed to recover Bacillus thuringiensis from soils and floral samples collected in the coastal area of the L_tzow-Holm Bay of the Antarctica sub-continent.(4) Characterization of the microsporidians, isolated from diseased larvae of the silkworm (Bombyx mori) in Brazilian sericultural farms, revealed that (i) the isolates with large- to middle-sized spores belong to the genus Nosema, but differ from the species Nosema bombycis, and (ii) the isolates with small-sized spores belong to the genus Pleistophora.

(1)基因序列分析表明，属于三个H血清型（福冈，sotto和galleriae）的五个鳞翅目特异性苏云金芽孢杆菌菌株的Cry蛋白，并产生球形伴孢包涵体，是单一类别，属于Cry9 δ-内毒素的新类别。(2)在1713株日本分离的苏云金芽孢杆菌中，有609株（35.5%）对小菜蛾幼虫表现出较高的杀灭活性。在这些毒性分离株中，605株属于H血清型1 - 10。当对属于4种H血清型（住吉虫，2株;福冈虫，1株;加拿大虫，1株;莫里索尼虫，1株）的5株选定高毒性分离株进行δ-内毒素检查时，除莫里索尼虫分离株外的4株分离株被分配为产Cry9D菌株。(3)分离实验未能从南极次大陆Ltzow-Holm湾沿海地区采集的土壤和花卉样品中回收苏云金芽孢杆菌。(4)从巴西养蚕场的病蚕（家蚕）中分离的微孢子虫的特征表明，（i）具有大至中等大小孢子的分离物属于微孢子虫属，但与家蚕微孢子虫种不同，（ii）具有小孢子的分离物属于Pleistophora属。

项目成果

Wasano,N.: "Delta-endotoxin proteins associated with spherical parasporal inclusions of the four Lepidoptera-specific Bacillus thuringiensis strains" Journal of Applied Microbiology. (印刷中). (1998)

Wasano, N.：“与四种鳞翅目苏云金芽孢杆菌菌株的球形伴孢内含物相关的 Delta 内毒素蛋白”《应用微生物学杂志》（1998 年出版）。

Higuchi,K.: "A novel isolate of Bacillus thuringiensis serovar leesis that specifically exhibits larvicidal activity against the moth-fly,Telmatoscopus albipunctatus" Systematic and Applied Microbiology. (印刷中). (1998)

Higuchi, K.：“苏云金芽孢杆菌李氏血清型的一种新分离株，对飞蛾、Telmatoscopus albipunctatus 具有杀幼活性”，《系统与应用微生物学》（1998 年出版）。

Wasano,N.: "Assignment of δ-endotoxin genes of the four Lepidoptera-specific Bacillus thuringiensis strains that produce spherical parasporal inclusions" Current Microbiology. 37・6. 408-411 (1998)

Wasano, N.：“产生球形伴胞内含物的四种鳞翅目苏云金芽孢杆菌菌株的 δ-内毒素基因的分配”《当代微生物学》37·6 (1998)。

Ohba,M.: "Identification of flagellar(H)antigenic subfactors in Bacillus thuringiensis H serotypes 10,18,and 24 isolated in Japan" Lett.Appl.Microbiol.23・5. 287-289 (1996)

Ohba, M.：“在日本分离的苏云金芽孢杆菌 H 血清型 10,18 和 24 中鞭毛 (H) 抗原亚因子的鉴定”Lett.Appl.Microbiol.23・5 (1996)。

Saitoh,H.: "Toxicity of Bacillus thuringiensis strains,naturally occurring in Japan,against larval Anopheles Stephensi(Diptera:Culicidae)" Med.Vet.Entomol.(印刷中). (1997)

Saitoh, H.：“日本天然存在的苏云金芽孢杆菌菌株对按蚊幼虫（双翅目：蚊科）的毒性”Med.Vet.Entomol.（出版中）。