Regulatory mechanism of various dietary factors affecting lipid and fatty acid metabolisms

本研究的目的是阐明芝麻素和表芝麻素对人体必需脂肪酸，特别是花生四烯酸（AA，n-6）、二十碳五烯酸（EPA，n-3）和二十二碳六烯酸（DHA，n-3）的体内滞留和代谢的调节作用。首先建立了高效液相色谱法同时测定芝麻素和表芝麻素含量的方法。然后，测定芝麻素和表芝麻素的体内代谢。它们在体内易于代谢，24小时后消失，提示它们的代谢产物具有调节必需脂肪酸代谢的功能。在分别给予AA、EPA或DHA后，观察到这些脂肪酸在组织中的积累，但芝麻素和episesamin降低了它们在各种组织中的浓度，并保持了脂肪酸浓度的稳态。因此，芝麻素和表芝麻素及其代谢产物将作为n-6和n-3系列必需脂肪酸代谢的调节物质。

The purpose of this research is to elucidate the regulatory functions of sesamin and episesamin from sesame seeds against retention in vivo and metabolism of essential fatty acids, especially arachidonate (AA,n-6), eicosapentaenoate (EPA,n-3) and docosahexaenoate (DHA,n-3). At first simultaneous determination method of sesamin and episesamin was established using HPLC. Then, metabolism of sesamin and episesamin in vivo was measured. They were easily metabolized in vivo and diappeared up to 24 hr. This result suggested that their metabolites function for regulation of metabolism of essential fatty acids. After administration of AA, EPA or DHA, respectively, accumulation of these fatty acids in the tissues was observed, but sesamin and episesamin reduced their concentrations in various tissues and retained the homeostasis of fatty acid concentrations. So, sesamin and episesamin including their metabolites will act as regulatory substances for metabolism of essential fatty acids of both n-6 and n-3 series.