Distribution of CaR in brain and its role

CaR在脑中的分布及其作用

• 批准号: 08457015
• 负责人: SORIMACHI Masaru
• 金额: $ 3.97万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457015/
• 关键词: monkey brain; CaR; spontaneous current; immunohistochemistry; Ca^<2+>感受性受容体

Our purpose of this study was to clarify the physiological role of Ca2+-sensing receptor (CaR) in the brain. We at first investigated the distribution of CaR in Japanese monkey brain using antibody against CaR.Only one kind of antibody against CaR fusion protein exhibited immunoreactivity in the brain. Immunoreactive beads-like nerve terminals were scattered in various brain regions including cerebra'neocortex, hippocampus, dentate gyrus, nucleus caudalis (faced to lateral vantricle), lateral and medial septum, striae terminalis, Meynert nucleus, amygdala area, thalamus (faced to lateral ventricle), hypothalamus (faced to third vantricle), midbrain interpeduncular nucleus, cerbellar cortex (surrounding Purkinje neuron), pons and medulla (particularly in area postrema). On the other hand, unlike the results in rat brain, we found no immunoreactivity on the wall of vessels. We next investigated the effect of antibody on the spontaneouus ionic cuurrents recorded in amygdala neurons to see whether CaR is involved in synaptic transmission. Application of antibody did not show any effect on ionic currents, suggesting that antibody did not recognize native CaR or uunaffected the spontaneous release of neurotransmitter from nerve terminals.

我们这项研究的目的是阐明 Ca2+ 传感受体 (CaR) 在大脑中的生理作用。我们首先使用抗CaR抗体研究了日本猴脑中CaR的分布。只有一种抗CaR融合蛋白的抗体在脑中表现出免疫反应性。免疫反应性珠状神经末梢散布在大脑新皮质、海马、齿状回、尾核（面向侧脑室）、外侧和内侧隔膜、终纹、Meynert核、杏仁核区、丘脑（面向侧脑室）、下丘脑等脑区 （面向第三脑室）、中脑脚间核、小脑皮质（周围浦肯野神经元）、脑桥和延髓（特别是后区）。另一方面，与大鼠脑中的结果不同，我们发现血管壁上没有免疫反应性。接下来我们研究了抗体对杏仁核神经元记录的自发离子电流的影响，以了解 CaR 是否参与突触传递。抗体的应用没有显示出对离子电流的任何影响，表明抗体不识别天然CaR或不影响神经末梢自发释放神经递质。

项目成果

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Jin, Y.-H.、Munakata, M.、Morimoto, H. 和 Akaike, N.：“苯二氮卓受体激动剂对与大鼠新纹状体急性分离的神经元的影响” NeuroRep.8。

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Sorimachi,M.,Rhee,J.S.等人：“GABA 和甘氨酸诱导鸡胚睫状神经节细胞胞质 Ca^2 浓度增加的机制”。

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Murai，Y.，Ishibashi，H.，等人：“由实验性缺血、缺氧和低血糖诱导的大鼠蓝斑神经元中Ca 2+ 激活的K 2 电流”，J.Neurophysiol.78。

Jin, Y.-H., Munakata, M.et al.: "Efects of benzodiazepine receptor agonists in neurones acutely dissociated from the rat neostriatum." NeuroRep.8. 3197-3200 (1997)

Jin, Y.-H., Munakata, M.等人：“苯二氮卓受体激动剂对与大鼠新纹状体急性分离的神经元的影响。”