Effects of interleukin-1beta on neurons in mammalian parasympathetic ganglia.

白细胞介素-1（IL-1）是一种细胞因子，在炎症、代谢、造血和免疫状态中具有广泛的活性。虽然IL-1 β可能调节自主神经功能并影响各种外周器官的应激处理，但IL-1 β对外周自主神经元功能的影响尚未在体外研究。本研究的目的是研究白细胞介素-1 β（IL-1 β）对离体哺乳动物盆神经节和牛蛙交感神经节神经元膜和突触传递的影响。连续应用重组人IL-1 β（6 pM-300 nM）10 s，在大鼠主要盆神经节（MPG）的11个神经元中产生了与输入电阻降低相关的持久超极化。在其他8个神经元中，IL-1 β产生由初始去极化和持续的超极化组成的双相反应。TTX（3 μ M）不能阻断这些对IL-1 β的反应。活性片段 关于我们 IL-1 β（IL-1 β 163-171，10-100 pM），人IL-1 β的活性结构域，模拟IL-1 β的作用。印防己毒素（100 μ M）不仅抑制初始去极化，而且抑制由IL-13 b（300 nM）诱导的超极化。这些结果提示γ-氨基丁酸（GABA）通过GABA_c受体介导IL-1 β产生的双相反应。由于db-环磷酸鸟苷（GMP）产生了一个初始的去极化，然后是一个持久的超极化，环GMP可能介导，至少部分，在大鼠MPG神经元IL-1 β诱导的超极化。IL-1 β对刺激兔膀胱盆神经节（VPG）诱发的EPSP有一个先易化后长时程抑制的过程。IL-1 β不影响乙酰胆碱（ACh）产生的去极化反应，表明IL-1 β减少了ACh从盆神经末梢的释放。IL-1 β可能抑制膀胱和其他盆腔器官的功能。少

Interleukin-1 (IL-1) is a cytokine which possesses a wide range of activities in inflammatory, metabolic, haemopoietic and immunological states. Although it is likely that IL-1beta modulates autonomic functions and affect stress processing in various peripheral organs, the effect of IL-1beta on the function of peripheral autonomic neurons is not investigated, in vitro. The aim of the present study was to examine the effect of interleukin-1beta (IL-1beta) on neuronal membrane and synaptic transmission in mammalian pelvic ganglia and bullfrog sympathetic ganglia, in vitro. Bath-application of recombinant human IL-1beta (6 pM-300 nM) for 10 s produced a long-lasting hyperpolarization associated with decreased input resistance in 11 neurons rat major pelvic ganglia (MPG). In other 8 neurons, IL-1beta produced a biphasic response which consists of an initial depolarization followed by a long-lasting hyperpolarization. TTX (3 muM) did not block these responses to IL-1beta. Active fragment of … More IL-1beta (IL-1beta 163-171, 10-100 pM), he active domain of human IL-l(beta, mimicked the effects of IL-1beta. Picrotoxin (100 muM) depressed not only initial depolarization but also hyperpolarization induced by IL-13b (300 nM). These results suggest that yaminobutyric acid (GABA) mediates the biphasic response produced by IL-1beta via GABA_c receptors. Since db-cyclic guanosine monophosphate (GMP) produced an initial depolarization followed by a long-lasting hyperpolarization, cyclic GMP might mediate, at least in part, the IL-1beta-induced hyperpolarization in rat MPG neurons. IL-1beta caused an initial facilitation followed by a long-lasting depression of the EPSP in rabbit vesical pelvic ganglia (VPG) evoked by stimulation of the pelvic nerve. TL-1beta did not affect the depolarizing response produced by acetylcholine (ACh), suggesting that IL-1beta reduces the release of ACh from the pelvic nerve terminals. It is likely that IL-1beta may inhibit the functions of the urinary bladder and other pelvic organs. Less