Study on abnormality in growth regulation hepatic carcinogenesis and its control.

肝癌生长调节异常及其防治研究。

• 批准号: 08457166
• 负责人: KAWATA Sumio
• 金额: $ 2.37万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457166/
• 关键词: hepatocellular cacinoma; TGF-beta receptor; cell growth; 細胞増殖

We have studied molecular mechanisms of human hepatocarcinogenesis from the view of the point that hepatocytes have an autonomous proliferative potential due to a loss of the responsiveness to a potent negative growth regulator, TGF-beta. Previously, we found poor response of human hepatoma-derived cell lines to TGF-beta.Hepatocellular carcinoma (HCC) occurs in the liver with HBV-or HCV-related cirrhosis. We introduced HBx gene into mink lung epithelial cell, a cell line which is very sensitive to TGF-beta. The responsiveness to TGF-beta in the transfectants was much poorer than that in the parent cells. In addition, the reduced responsiveness seemed to result from a decrease in expression of TGF-beta receptors. The hepatocytes could escape from the negative growth regulation by TGF-beta due to expression of HBx gene during chronic HBV infection.

我们已经研究了人类肝癌发生的分子机制，从肝细胞具有自主增殖的潜力，由于一个强大的负性生长调节因子，TGF-β的反应性的损失的观点。以前，我们发现人肝癌细胞系对TGF-β的反应很差。肝细胞癌（HCC）发生在HBV或HCV相关肝硬化的肝脏中。我们将HBx基因导入水貂肺上皮细胞，这是一种对TGF-β非常敏感的细胞系。转染细胞对TGF-β的反应性比亲本细胞差得多。此外，反应性降低似乎是由于TGF-β受体表达减少所致。慢性HBV感染时，肝细胞可通过HBx基因的表达逃避TGF-β的负性生长调节。

项目成果

N.Ito, S.Higashiyama, S.Kawata, et al.: "Regulation of heparin-binding EGF-like growth factor expression by phorbol ester in a human hepatoma-derived cell line" Biochim.Biophys.Acta. 1310. 163-167 (1996)

N.Ito、S.Higashiyama、S.Kawata 等人：“在人肝癌细胞系中佛波酯对肝素结合 EGF 样生长因子表达的调节”Biochim.Biophys.Acta。

H.Tsushima, S.Kawata, et al.: "HIGH LEVELS OF TGF-beta 1 in patients with colorectal cncer : Association with disease progression" Gastroenteology. 110(2). 375-382 (1996)

H.Tsushima、S.Kawata 等人：“结直肠癌患者中高水平的 TGF-β1：与疾病进展的关联”胃肠病学。

H.Tsushima,S.Kawata,et al.: "High levels of TGF-β1 in patients with colorectal cacer : Association with disease progression." Gastroenterology. 110(2). 375-382 (1996)

H. Tsushima、S. Kawata 等人：“结直肠癌患者中高水平的 TGF-β1：与疾病进展的关系”110(2) (1996)。

O. Oshikawa, S. Tamura S. Kawata, et al.: "The effect of hepatitis B virus gene expression on response to growth inhibition by TGF-β1" Biochem. Biophys. Res. Cpmmun.222 (3). 770-773 (1996)

O. Oshikawa、S. Tamura S. Kawata 等人：“乙型肝炎病毒基因表达对 TGF-β1 生长抑制反应的影响”Biochem.Cpmmun.222 (3)。 (1996)

N. Ito, S. Kawata, et al.: "Increased circulating TGF-β1 in a patient with giant hepatic hemangioma ; Possible contribution to impaied immune tunction." Hepatology. 25 (1). 93-96 (1997)

N. Ito、S. Kawata 等人：“巨大肝血管瘤患者循环 TGF-β1 增加；可能导致免疫功能受损。”25 (1)。