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Morphometric Studies of the Brains of Alzheimer's Disease by Computer Image Analyzer.

通过计算机图像分析仪对阿尔茨海默氏病大脑进行形态测量研究。

基本信息

批准号：
08457249
负责人：
MIYOSHI Koho
金额：
$ 4.93万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Morphometric investigations revealed that temporal and occipital lobe were most severely degenerated in the cerebral cortex of Alzheimer's disease.The brains from 5 AD patients and 5 age-matched brains from the case with normal aging were studied morphometrically with computer image analyzing system. Serial paraffin sections of 10 microns were prepared from the tissues of the cerebral cortex, and stained with Nissl for counting neurons, Bodian for neurofibrillary tangles, and beta-protein for senile plaques.The diminution rates of total neurons in the examined areas were as follows : orbital gyrus (32.0%), precentral gyrus (18.0%), inferior temporal gyrus (43.0%), medial temporal gyrus (44.0%), superior temporal gyrus (58.0%), parietal cortex (45.0%), calcarine area of the occipital lobe (52.0%), and hippocampus (48.0%). Large sized neurons diminished remarkably in the cerebral cortex. The severity of neuronal loss of neruons was different in each cortical layer. For example, in the inferior temporal cortex, the neuronal diminution rates of the cortical layers were 18.6%, 17.8%, 9.3% in the 3rd, 4th, and 5-6th layers, respectively. The diminution rates of the neurons in the cortical layers were had direct correlation with the number of neurofibrillary tangles, but did not have positive correlation with the number of senile plaques. Most distinguished neuronal losses were observed in the 5-6th layer of the superior temporal gyrus, and in 3rd layer of the inferior temporal gyrus.

形态计量学研究显示阿尔茨海默病患者大脑皮质的颞叶和枕叶变性最为严重。应用计算机图像分析系统对5例AD患者和5例正常衰老患者的大脑进行了形态计量学研究。取大脑皮质组织制作连续的10微米石蜡切片，尼氏染色计数神经元，Bodian染色神经原纤维缠结，β-蛋白染色老年斑。受检区神经元减少率依次为：眶回(32.0%)、中央前回(18.0%)、颞下回(43.0%)、内侧回(44.0%)、颞上回(58.0%)、顶叶皮质(45.0%)、枕叶距骨区(52.0%)和海马区(48.0%)。大脑皮层大型神经元明显减少。各皮质层神经元丢失的严重程度不同。以颞叶下部皮质为例，第3、4、5~6层皮质神经元减少率分别为18.6%、17.8%、9.3%。皮质层神经元减少率与神经原纤维缠结数呈正相关，与老年斑块数无正相关。最明显的神经元丢失见于颞叶上回的5~6层和颞下回的第3层。