Cell culture study on the mechanism and control of late graft failure occuring in arterial reconstruction procedures.

动脉重建过程中发生晚期移植失败的机制和控制的细胞培养研究。

• 批准号: 08457306
• 负责人: KAMADA Yoshihiko
• 金额: $ 4.1万
• 依托单位: Ryukyu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457306/
• 关键词: autovein graft; endothelial cell; smooth muscle cell; intimal hyperplasia; shear stress; collagen; cell culture; vascular surgery; 細胞培養; 術後晩期閉塞; 仮性内膜肥厚; 内皮細胞; 末梢血管抵抗

The mechanism causing intimal hyperplasia at the anastomotic site in peripheral revascularization procedures, resulting in late graft failure, is obscure at the present time. This intimal hyperplasia occurs mostly in grafts with poor distal outflow, at regions of low shear stress. We studied the effects of shear stress on type IV and type IV collagen production by cultured canine vascular endothelial cells, using disk a rotation device. Semi-quantitative measurements on immunohistochemically processed samples were performed. We found that lower shear stress induced the endothelial cells to produce more type I and type IV collagen. Type IV collagen is a major component of the subendothelial basement membrane. Type I collagen occurs throughout the depth of the hyperplastic intima, and has been shown to promote modulation of cultured arterial smooth muscle cells from a contractile to a synthetic phenotype. We hypothesize that vascular endothelial cells can regulate subendothelial smooth muscle cell activity through control of collagen production in response to shear stress conditions. This mechanism might play an important role in the development intimal hyperplasia seen in the late postoperative period.

外周血运重建术中吻合口内膜增生导致移植物晚期失效的机制目前尚不清楚。这种内膜增生主要发生在远端流出不良的移植物中，在低剪切应力区域。本研究采用圆盘旋转装置，研究了剪切力对培养的犬血管内皮细胞产生IV型胶原和IV型胶原的影响。半定量测量化学处理的样品进行。我们发现，较低的剪切力诱导内皮细胞产生更多的I型和IV型胶原。IV型胶原是内皮下基底膜的主要成分。I型胶原蛋白存在于增生内膜的整个深度，并且已经显示出促进培养的动脉平滑肌细胞从收缩表型到合成表型的调节。我们假设血管内皮细胞可以通过控制胶原蛋白的产生来调节内皮下平滑肌细胞的活性。这种机制可能在术后晚期内膜增生的发展中起重要作用。

项目成果

Ohta O,Kusaba A.: "Development of vasa vasorum in the arterially implanted autovein bypass graft and its anastomosis in dogs." Int Angiol. 16(3). 197-203 (1997)

Ohta O，Kusaba A.：“犬动脉植入自静脉旁路移植物中滋养管的发育及其吻合术。”

佐久田斉、草場昭、鎌田義彦、他: "膝下病変に対する血行再建術の検討" 日本血管外科学会誌. 4(5). 29-35 (1996)

Hitoshi Sakuta、Akira Kusaba、Yoshihiko Kamata 等：“膝关节以下病变的血运重建研究”日本血管外科学会杂志 4(5) (1996)。

Kawabata T,Shiroma H,Koja K,Kusaba A.: "Pathophysiological features of intimal hyperplasia of the arterially implanted autovein graft and its anastomosis in dogs. Immunohistochemical detection using 5-bromo-2-deoxyuridine." Int j Angiol. (accepted). (1997

Kawabata T、Shiroma H、Koja K、Kusaba A.：“犬动脉植入自血管移植物内膜增生及其吻合的病理生理学特征。使用 5-溴-2-脱氧尿苷进行免疫组织化学检测。”

草場昭、佐久田斉、鎌田義彦、他: "人工血管感染に対する制御対策の工夫" 日本血管外科学会誌. 6(4). 463-472 (1997)

Akira Kusaba、Hitoshi Sakuta、Yoshihiko Kamata 等：“人工血管感染控制措施的改进”日本血管外科学会杂志 6(4) (1997)。

佐久田斉,草場昭,鎌田義彦,他: "膝下病変に対する血行再建術の検討" 日本血管外科学会誌. 4(5). 29-35 (1996)

Hitoshi Sakuta、Akira Kusaba、Yoshihiko Kamata 等：“膝关节以下病变的血运重建研究”日本血管外科学会杂志 4(5) (1996)。