Monecular aspect of cholesterol metabolism in gallstone formation

1.人胆固醇7 α-羟化酶的测定当我们在溶解微粒体并理想地重建P450反应系统后测量微粒体胆固醇7 α-羟化酶活性时，没有测定微粒体胆固醇7 α-羟化酶活性作为增加的测量;而线粒体胆固醇27-当我们在溶解线粒体和重建P450反应系统后测量羟化酶活性时，羟化酶活性被测定为显著增加的测量理想情况下，结果将被公布。胆固醇和维生素D_3的代谢胆固醇27-羟化酶和维生素D_3 25-羟化酶是由一种共同的酶来完成的，为了考察这些现象，我着重研究了与维生素D_3有关的酶。首先，我们从大鼠肾脏线粒体中分离纯化了25-羟维生素D_3 1 α-羟化酶，并发表了两篇论文，该酶的底物特异性有待进一步研究。

1. Assay of human cholesterol 7alpha-hydroxylaseMicrosomal cholesterol 7alpha-hydroxylase activity was not assayed as an increased measurement when we measured it after solubilizing the microsomes and reconstituting the P450 reaction system ideally ; whereas mitochondrial cholesterol 27-hydroxylase activity was assayed as a markedly increased measurement when we measured it after solubilizing the mitochondria and reconstituting the P450 reaction system ideally.The results will be published.2. Cholesterol and vitamin D_3 metabolismsCholesterol 27-hydroxylase and vitamin D_3 25-hydroxylase are performed by one common enzyme.To survey these phenomena, I focused to study enzymes concerning vitamin D_3. First, we purified 25-hydroxyvitamin D_3 1alpha-hydroxylase from rat kidney mitochondria, and published as two papers.Substrate specificity of the enzyme will be studied in future.