Trearment for DIND and DND after subarachnoid hemorrhage

蛛网膜下腔出血后 DIND 和 DND 的治疗

• 批准号: 08457371
• 负责人: AIHARA Noritaka
• 金额: $ 5.06万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457371/
• 关键词: SAH; apoptosis; immediate early gene; CA1; hypothermia; bax; bcl-2; アポトーシス; 脳血管萎縮; Subarachnoid hemorrhege; rat; vasospasm; Delayad Nearoncl Death

We have detected c-fos and c-jun mRNA in cerebral cortex, hippocampus and dentate gyrus in the penetrated side after subarachnoid hemorrhage (SAH). mRNA coding for hsp7O was induced in the cerebral cortex, hippocampus, thalamus, hypothalamus, and caudoputamen in the penetrated side and extended to the contralateral side. In the TUNEL.method, only CAl neurons in the penetrated side showed apoptosis-like changes 48hr after SAH.The genes for bax and p53 were induced fairly in the CAl with peak between l2hr and 48hr which might cause apoptosis, although bcl-2 was also upregulated during the same period. The regulation of bax/bcl-2 ratio through p53 might influence apoptotic cell death. We investigated the effect of hypothermia on brain exposed to SAH.Mild hypothermia depressed hsp7O mRNA expression in the cortex, thalamus and hippocampus. The c-jun mRNA expression was also reduced with hypothermia in the cortex, thalamus and CA1. In the future, we indicated that hypothermia therapy might be one of the procedures to treat SAH.

本实验检测了蛛网膜下腔出血（SAH）后大鼠大脑皮质、海马和齿状回c-fos和c-jun mRNA的表达。Hsp 7 O mRNA在穿透侧的大脑皮质、海马、丘脑、下丘脑和尾壳核中被诱导表达，并延伸到对侧。TUNEL法显示，SAH后48小时，仅穿通侧CA 1区神经元出现凋亡样改变，bax和p53基因在CA 1区有较强的诱导表达，在12 ~ 48小时达高峰，bcl-2基因在此期间也有上调表达，可能是引起凋亡的原因。p53对bax/bcl-2比值的调节可能影响细胞凋亡。我们研究了低温对SAH脑组织的影响，亚低温降低了皮质、丘脑和海马中hsp 7 O mRNA的表达。c-jun mRNA的表达也降低与低温在皮层，丘脑和CA 1。亚低温治疗有望成为治疗SAH的方法之一。

项目成果

神谷 健: "クモ膜下出血" 総合臨床. 46(1). 109-113 (1997)

Ken Kamiya：“蛛网膜下腔出血”《一般临床实践》46（1）109-113（1997）。

Masao Nakatsuka, Atsuo Masago, Kazuo Yamada, Hideaki Taki, Shigenori Harada: "Relationship between the expression of p53 mRNA and apoptosis in the hippocampus in the early phase after subarachnoid hemorrhage" Proceedings of 13th Spasm Symposium in Kyoto.

Masao Nakatsuka、Atsuo Masago、Kazuo Yamada、Hideaki Taki、Shigenori Harada：“蛛网膜下腔出血后早期海马 p53 mRNA 表达与细胞凋亡的关系”京都第 13 届痉挛研讨会论文集。

山田和雄・神谷健: "脳神経外科レビュー(4)" 三輪書店, 74-88 (1996)

Kazuo Yamada 和 Ken Kamiya：“神经外科评论（4）” Miwa Shoten，74-88（1996）

馬為衆ら: "ラットくも膜下出血モデルによって海馬病変は引き起こされるか?" 第23回日本脳卒中学会 抄録集. 108 (1998)

Tameshu Uma 等人：“大鼠蛛网膜下腔出血模型会导致海马病变吗？”第 23 届日本中风学会会议记录 108 (1998)。

中塚雅雄ら: "ラットくも膜下出血後早期の海馬におけるp53mRNAの発現とアポトーシスの関連について" 脳血管攣縮. 13. 261-266 (1998)

Masao Nakatsuka 等：“蛛网膜下腔出血后早期大鼠海马 p53 mRNA 表达与细胞凋亡的关系”Cerebral Vasospasm 13. 261-266 (1998)。