Fluormetric Study on interaction of Media Having Internal Hadrophobic Environment with Analytical Reagents

根据提出的项目进行了一些实验，得到了以下结果：用荧光法研究了黄酮和香豆素与-环糊精（CD）在有机水溶液中的包合平衡。考虑到- cd中所含的有机溶剂被这些荧光试剂取代，可以分析表观包合常数与有机溶剂浓度的关系。由此估计的- cd包合有机溶剂的常数与相应有机溶剂的溶解度参数相关。分析了流动相β - cd浓度对RPLC柱上黄酮、色酮和香豆素容量因子的影响。这些试剂在色谱柱上的保留可以通过考虑流动相中含有β - cd的试剂来定量解释。讨论了试剂的结构特性对固定相与流动相分离的影响。分析了黄酮类化合物与阳离子和中性表面活性剂胶束的相互作用及其胶束浓度的关系。试剂与胶束的结合常数可以通过考虑反应物之间的静电和疏水相互作用来解释。通过与β - cd体系的结果比较，讨论了影响缔合平衡和荧光增强的因素。

Under the projects proposed some experiments were carried out and the following results were obtained.1. Inclusion Equilibria of flavones and coumarins with beta-cyclodextrin(CD) have been studied fluorometrically in aqueous organic solvents. Dependence of the apparent inclusion constants on the concentration of organic solvent could be analyzed by taking the substitution of organic solvent included in beta-CD with these fluorometric regents into consideration. The constants thus estimated for the inclusion of organic solvent with beta-CD were found to be correlated with solubility parameter of respective organic solvents.2. Capacity factor for flavones, chromons and coumarins on RPLC column has been analyzed as a function of the concentration of beta-CD in mobile phase. The retention of these reagents on the column could be quantitatively interpreted by taking the inclusion of the reagents with beta-CD in mobile phase into consideration. Effect of structural characteristics of the reagents on their partiton between stationary phase and mobile phase was discussed.3. Interraction of flavones with cationic and neutral surfactant micelles has been analyzed as a function of the concentration of respective micelles. Association constant of the reagents with the micelles could be interpreted by taking the electrostatic and the hydrophobic interactions between the reactants into consideration. By comparing the present results with those obtained in beta-CD system, factors affecting the association eqilibria and the resulting fluorescence enhancement were discussed.