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Fluormetric Study on interaction of Media Having Internal Hadrophobic Environment with Analytical Reagents

内部疏水环境介质与分析试剂相互作用的荧光研究

基本信息

批准号：
08650961
负责人：
YAMADA Shinkichi
金额：
$ 1.28万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08650961/
关键词：
Cyclodextrin Surfactant Fluorometric reagent Inclusion reaction

项目摘要

Under the projects proposed some experiments were carried out and the following results were obtained.1. Inclusion Equilibria of flavones and coumarins with beta-cyclodextrin(CD) have been studied fluorometrically in aqueous organic solvents. Dependence of the apparent inclusion constants on the concentration of organic solvent could be analyzed by taking the substitution of organic solvent included in beta-CD with these fluorometric regents into consideration. The constants thus estimated for the inclusion of organic solvent with beta-CD were found to be correlated with solubility parameter of respective organic solvents.2. Capacity factor for flavones, chromons and coumarins on RPLC column has been analyzed as a function of the concentration of beta-CD in mobile phase. The retention of these reagents on the column could be quantitatively interpreted by taking the inclusion of the reagents with beta-CD in mobile phase into consideration. Effect of structural characteristics of the reagents on their partiton between stationary phase and mobile phase was discussed.3. Interraction of flavones with cationic and neutral surfactant micelles has been analyzed as a function of the concentration of respective micelles. Association constant of the reagents with the micelles could be interpreted by taking the electrostatic and the hydrophobic interactions between the reactants into consideration. By comparing the present results with those obtained in beta-CD system, factors affecting the association eqilibria and the resulting fluorescence enhancement were discussed.

在提出的方案下进行了实验研究，取得了以下成果.用荧光光谱法研究了黄酮类化合物和香豆素类化合物与β-环糊精（CD）的包结平衡。通过考虑这些荧光试剂对β-CD中所含有机溶剂的取代，可以分析表观包结常数对有机溶剂浓度的依赖性。由此估计的有机溶剂与β-CD包结的常数与相应有机溶剂的溶解度参数相关.分析了黄酮类、色酮类和香豆素类化合物在RPLC柱上的容量因子与移动的相中β-CD浓度的关系。这些试剂在柱上的保留可以通过考虑在移动的相中包含具有β-CD的试剂来定量解释。讨论了试剂的结构特征对其在固定相和移动的相间分配的影响.黄酮与阳离子和中性表面活性剂胶束的相互作用已被分析作为各自胶束浓度的函数。结合常数可以通过考虑反应物之间的静电相互作用和疏水相互作用来解释。通过与β-CD体系的比较，讨论了影响缔合平衡的因素及荧光增强效应。