Alleviation of Chilling injury of fruits and vegetables by heat treatment and molecular biological study on its mechanism

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Cucumber fruit applied heating treatment ; immersing into hot water (for 5-90 minutes at 45-50ﾟC), was retarded the increase in the rate of ion leakage from flesh section. If the fruit were held at 20ﾟC after heating , these fruits tended to loose chilling tolerance obtained by heat treatment. Although the overall protein pattern as seen by Coomassie brilliant blue or silver staining was similar from heated and unheated fruit, some low-molecular-mass proteins (14-18kDa) were pbserved in the heated fruit that were absent or present in reduced amounts in unheated fruit. Heat shock (i.e., 60 min.at 40ﾟC) also induces chilling tolerance for 24h at 1ﾟC in cucumber seedlings.It is especially effective for radicle elongation and development of lateral roots, however, they decreased with prolonged chilling. The heat treatment for seedlings would allow them to be sowed at lower temperatures than is currently feasible.