Studies on Reproduction and Age Determination of Deep-sea Squalidae, Deania spp.

1.雄性全年进行精子发生。2.两种雄性都没有明确的生殖周期和交配季节。3.雌性在子宫中携带6-24个胚胎。同一月份采集的雌体胚胎大小不一，因此雌体也没有明显的交配、排卵和分娩季节。4.雌雄体的最小成熟尺寸分别为650 mm TL和822 mm TL，雌体的最小成熟体大小分别为750 mm和1020 mm。5.受精卵的湿重含水率(FE)在50%~55%之间，而胚胎中的含水率随着发育而线性增加到70%。6.外部卵黄囊小于100 mm TL的胚胎的干重几乎与FE相同，7.胚胎肝脏干重在胚胎发育到105 mm后开始急剧增加。8.母血血浆蛋白浓度随胚胎…变化不大胎龄为16 mg/ml，75 mm胚胎约为7 mg/ml，140 mm以上胚胎小于2.5 mg/ml。子宫液谱显示，从FE到105 mm胚胎的发育阶段有40-60kd的蛋白质。10.在105 mm以上的胚胎肠腔内观察到卵黄物质，两种鱼的脊骨表面均无生长带。12.石斑鱼的棘长与体长呈线性增长，相关系数达极显著水平。D的体长与体长的协方差高，相关系数低。13.脊柱由外套膜、突出牙本质部分、茎、髓腔、软骨组织5个结构组成。14.茎内牙本质层有生长环。新的生长环形成于内牙本质层内。15.生长环的数量随个体大小呈线性增加。16.金龟子和水生金龟子分别有1-15环和6-15环。17.模式为雄性金龟子第6环，雌性金龟子第10环，雄性金龟子第9环，雌性金龟子第13环。18.不能证实每年是否形成生长环。较少

1.Male performed spermatogenesis through the year.2.Males of both species did not have a clear reproductive cycle and copulative season.3.Female carried 6 to 24 embryos in her uteri. Females collected in the same month had various size of embryos, so females also have no distinct copulative, ovulatory and delivery seasons.4.Minimum mature size of male and female Deania calcea was 650 mm TL and 822 mm TL,respectively, and in male and female D.hystricosa was 750 mm and 1020 mm, respectively.5.Water content in wet weight of fertilized eggs (FE) ranged from 50 to 55%, while that in the embryos increased lineally to 70 % with their growth.6.Dry weight of the embryos with external yolk sac smaller than 100 mm TL was almost equal to that of FE,but the embryos larger than 100 mm tended to be light with their growth.7.Dry weight of the liver in the embryos began to increase suddenly after embryos had grown to 105 mm.8.Protein concentration of maternal blood plasma hardly changed with embryonic … More growth, whiie that of uterine fluid decreased ; about 16 mg/ml to FE,7mg/ml to the 75 mm embryo, and less than 2.5 mg/ml to the embryos over 140 mm.9.Uterine fluid profiles showed proteins of 40-60 kd specifically at the developmental stages from FE to the 105 mm embryo. Proteins of 14-40 kd and 60-79 kd were observed at every stage.10.Yolk matter was observed ih the intestinal lumen of the embryos over 105 mm.11.The spines of both species had no growth band on their surface.12.The spine length in D.calcea increased lineally with body size and their correlation coefficients were high significant. In D,hystricosa, the covariance between body size and spine length was high and their correlation coefficients were low.13.The spine consisted of five structural components ; mantle, protruding dentine portion, stem, pulp cavity, cartilaginous tissue.14.Growth rings were observed on the inner dentine layr of stem. The newly growth ring was formed inside the inner dentine layr.15.The number of growth rings increased lineally with body size. Small D.calcea had one ring.16.D.calcea and D.hystrlcosa had 1-15 and 6-15 rings, respectively.17.Mode was 6th ring in male D.calcea, 10th ring in female D.calcea, 9th ring in male D.hystricosa, and13th ring in female D.hystricosa.18.We could not verify that growth ring is formed annually. Less