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Effect protein deficiency on lnsulin like growth factor-I and lnsulin like growth factor-II gene expression physiological reles of lnsulin like growth factors as a local factore in growing lambs-

蛋白质缺乏对胰岛素样生长因子-I和胰岛素样生长因子-II基因表达的影响胰岛素样生长因子作为生长羔羊局部因子的生理关系-

基本信息

批准号：
08660343
负责人：
MATSUI Tohru
金额：
$ 1.41万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

To study the mechanism of relationship between protein nutrition and bone growth, growing lambs were fed protein deficient diet or control diet and the gene expression of lnsulin like growth factor-I and lnsulin like growth factor-II were measured by RT-PCR in the growth cartilage. Additionally plasma concentrations of lnsulin like growth factor-I and lnsulin like growth factor-II were determined by dadio immunoassay. Male castrated lambs weighing approximately 20 kg were allotted to two groups in a double reversal design. These animals were fed control diet (crude protein content ; 17.5%) or protein deficient diet (crude protein content, 4.9%) . Energy contents in these diets satisified for its requirement. Protein deficiency suppressed body weight gain. Plasma insulin like growth factor-I concentration was lowered When lambs were fed the protein deficient diet. Compared to lnsulin like growth factor-I, plasma lnsulin like growth factor-II concentration was remarkably low in each group. And plasma lnsulin like growth factor-II was not affected by protein deficiency. Transcript of lnsulin like growth factor-I in the growth cartilage tended to be reduced by protein deficiency though this change was not significant. However, transcript of lnsulin like growth factor-II in the growth cartilage was not affected by protein deficiency. These results suggested that protein deficiency suppressed bone growth through reducing plasma lnsulin like growth factor-I concentration and locally produced lnsulin like growth factor-I in the growth cartilage. However, plasma lnsulin like growth factor-II and locally produced lnsulin like growth factor-II were not related to the retardation of bone growth in protein deficient sheep.

为探讨蛋白质营养与骨生长关系的机理，采用RT-PCR方法检测生长期羔羊生长软骨中胰岛素样生长因子-I（Insulin-like growth factor-I）和胰岛素样生长因子-II（Insulin-like growth factor-II）基因的表达。此外，通过放射免疫测定法测定胰岛素样生长因子-I和胰岛素样生长因子-II的血浆浓度。将体重约20 kg的雄性去势羔羊按双反转设计分配至两组。这些动物饲喂对照饲料（粗蛋白含量; 17.5%）或蛋白质缺乏饲料（粗蛋白含量，4.9%）。这些日粮中的能量含量满足了其需要。蛋白质缺乏抑制体重增加。血浆胰岛素样生长因子-I浓度降低时，羔羊蛋白质缺乏的饮食。与胰岛素样生长因子-I相比，血浆胰岛素样生长因子-II浓度在各组中显著低。血浆胰岛素样生长因子-II不受蛋白质缺乏的影响。生长软骨中胰岛素样生长因子-I的转录水平在蛋白质缺乏时有降低的趋势，但这种变化并不显著。然而，生长软骨中胰岛素样生长因子-II的转录不受蛋白质缺乏的影响。这些结果表明，蛋白质缺乏通过降低血浆胰岛素样生长因子-I浓度和生长软骨中局部产生的胰岛素样生长因子-I来抑制骨生长。然而，血浆胰岛素样生长因子-II和局部产生的胰岛素样生长因子-II与蛋白质缺乏绵羊骨生长迟缓无关。