Cell-surface associated cxtracellular proteoglycan involved in migration of the cell

在嗅上皮、嗅神经和嗅神经中发现了对单克隆抗体呈免疫反应的神经蛋白聚糖（PG），鸡胚嗅神经束在胚龄（艾德）3-3.5天出现PG免疫反应，在ED 5 - 11天，神经束内的PG免疫反应逐渐增强，细胞表面的PG免疫反应也逐渐增强，细胞膜上的PG免疫反应也逐渐增强。免疫荧光双标记结果显示，PG在嗅神经中分布于含GnRH细胞和沿着嗅神经迁移的其它细胞的胞体表面，在电镜下，PG在嗅神经中的分布与嗅神经中的分布一致，在嗅神经中的分布与嗅神经中的分布一致。结果表明，鸡胚神经PG是一种细胞表面结合的细胞外基质分子，PG覆盖在迁移细胞和延长的嗅神经表面，PG在鼻区的细胞表面相关的细胞外定位，结合大鼠脑组织中PG与细胞粘附分子的结合特性，提示PG对鸡胚嗅神经细胞沿着迁移有一定的引导作用。

A neural proteoglycan (PG) immunoreactive to a monoclonal antibody was seen in the olfactory epithelium, the olfactory nerve, and the cells orignating the epithelium and migrating along the olfactory nerve toward the forebrain in chick embryos.Immunoreactivity to the PG appeared on embryonic day (ED) 3-3.5 in the bundles of the olfactory nerve.The immunoreactivity in the nerve bundles gradually increased during ED5-11.Cell surface-associated extracellular localization of the immunoreactivity was seen in the olfactory epithelium on ED6 and afterwards.Immunofluorescent double-labeling of the PG and gonadotropin-releasing hormone (GnRH) revealed that the PG covered the surface of the cell bodies of both GnRH-containing cells and other cells migrating along the olfactory nerve.Under an electron microscope, the PG was seen on the surfaces of the cell bodies and of the neurites in the nerve bundles.These results indicate that the neural PG in chick embryos is one type of cell surface-associated extracellular matrix molecule, and that the PG covered the surfaces of migrating cells and of elongating olfactory nerve.The cell surface-associated extracellular localization of the PG found in the nasal region, taken together with the binding properties of this PG with cell adhesion molecules shown in rat brains, suggested than the PG playd a role in guiding the migration of cells along the olfactory nerve in chick embryos.