Induction of Apoptosis to Rat Thyroid Follicular Epithelial Cell Line, FRTL-5 Cell

诱导大鼠甲状腺滤泡上皮细胞系 FRTL-5 细胞凋亡

• 批准号: 08670199
• 负责人: KAWAOI Akira
• 金额: $ 1.41万
• 依托单位: Yamanashi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670199/
• 关键词: Apoptosis; Thyroid; Bone marrow derived cell; 骨髄細細; 骨髄細胞; IL-3

In the present study TSH dependnt apoptosis was successfully induced to FRTL-5 cells by replacing conventional monolayr culture with suspension culture.Since cell to cell or cell to matrix adhesion was considered to be significantly weakenend in suspension culture, the adhesion molecules such as E-cadherin, integrins and their ligands such as laminin, type 4 collagen, fibronectin which are expressed in FRTL-5 cells were immunocytochemically examined, and their expression in the cells was suggested to be up-regulated by TSH in suspension culture.The possibility was deduced from above observations that induction of TSH dependent apoptosis in FRTL-5 cells was initiated by the decreased adhesiveness of cells caused by down regulated expression of adhesion molecules in these cells.The questions remained to be answered are as follows : 1. What is the role of TSH in the expression of adhesion molecules in monolayr cultured cells which are resistant against apoptosis? 2. Are there, if any, regulatory factors which make FRTL-5 cells resistant vs apoptosis by keeping cell adhesion stable in the condition of monolayr culture without TSH? 3. How TSH exerts its effects on the cell adhesion in FRTL-5 cells, and why the deprivation of TSH results in the loosening of cell adhesion and finally in apotosis?

本研究用悬浮培养法代替传统的单层培养法成功地诱导了FRTL-5细胞凋亡，由于悬浮培养法明显减弱了细胞与细胞或细胞与基质的粘附，因此免疫细胞化学方法检测了FRTL-5细胞表达的粘附分子如E-钙粘素、整合素及其配体如层粘连蛋白、4型胶原、纤连蛋白，结果表明，FRTL-5细胞中粘附分子的表达水平在悬浮培养中受到TSH的上调，由此推测，FRTL-5细胞中粘附分子的表达下调，导致细胞凋亡率下降，从而诱导TSH依赖性的细胞凋亡。在抗凋亡的单层培养细胞中，TSH在粘附分子表达中的作用是什么？2.在无促甲状腺激素的单层培养条件下，FRTL-5细胞是否存在通过维持细胞粘附稳定而抵抗凋亡的调节因素？3. TSH对FRTL-5细胞粘附的影响机制，以及TSH缺乏导致FRTL-5细胞粘附松动并最终导致细胞凋亡的原因。

项目成果

Tasaka.K.: "Histochemistry and Cytochemistry 1996" (1996)

Tasaka.K.：“组织化学和细胞化学 1996”（1996）

田坂捷雄: "アポトーシスを回避するモノクローン抗体のinvivoでの役割" 日本病理学会会誌. 84・1. 226- (1995)

Yoshio Tasaka：“单克隆抗体避免细胞凋亡的体内作用”日本病理学会杂志84・1。

K.TASAKA: "Histochemistry and Cytochemistry 1996"

K.TASAKA：“组织化学和细胞化学 1996”

田坂捷雄: "Histochemistry and Cytochemistry 1996" (1996)

田坂芳夫：“组织化学和细胞化学 1996”（1996）

K.TASAKA: "In vivi role of monoclonal antibody which can induce escape from apoptosis." Transactiones Societatis Pathologicae Japonicae.84 (1). 226 (1995)

K.TASAKA：“单克隆抗体在体内的作用可以诱导细胞凋亡的逃逸。”