Superconducting NMR Magnet 400 MHz

超导核磁共振磁体 400 MHz

• 批准号: 528284074
• 金额: --
• 依托单位: Martin-Luther-Universität Halle-Wittenberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/528284074?language=en
• 关键词: Superconducting; NMR; Magnet; 400; MHz

The key objective of the following proposal is a replacement of a superconducting magnet for the 400 MHz NMR spectrometer in the Department of Natural Product Biochemistry at the Institute of Biochemistry and Biotechnology of the Martin Luther University Halle-Wittenberg. The Department specializes in protein and natural products biochemistry and, in particular, addresses research questions in the directions of protein (re)folding and stabilization, enzyme design, peptide synthesis and medium engineering with help of NMR spectroscopy. The 400 MHz NMR spectrometer is an essential instrument in the group and of a central importance in the frames of ongoing and planned research projects aimed at elucidating structure-interaction relationships between highly tuneable ionic liquids and complex biomolecules such as peptides and small proteins, as well as studying kinetic of enzymatic reactions. To that end, the high-resolution multidimensional NMR experiments of both solutions and more challenging highly-viscous samples are necessary. With the established high-resolution magic angle spinning NMR toolbox such measurements were possible in the group. In the planned research projects this NMR technique will be further applied to monitor conformational changes and local structure fluctuations of various elongated structured peptides and small proteins upon dissolving them in ionic liquids, as well as to probe the influence of ionic liquids on highly stable structured biomolecules. In addition to structural features NMR spectroscopy will be used to examine dynamics of different ionic liquids as well as biomolecules dissolved in them by exploiting their relaxation properties. Accurate characterization of interactions between ionic liquids and biocatalysts or peptide and protein targets will facilitate the design of application-specific ionic liquids. Finally, NMR spectroscopy will be applied to address cleavage preferences of peptidases targeting various antimicrobial peptides and kinetic parameters of such enzymatic reactions will be derived. In addition the 400 MHz NMR-Spectrometer will be used for routine analysis measurements in MLU Halle.

以下提案的主要目标是更换马丁路德大学哈雷-维滕贝格生物化学和生物技术研究所天然产物生物化学系400 MHz NMR光谱仪的超导磁体。该部门专门从事蛋白质和天然产物生物化学，特别是解决蛋白质（重新）折叠和稳定，酶设计，肽合成和介质工程的帮助下，NMR光谱方向的研究问题。400 MHz NMR光谱仪是该团队的重要仪器，在正在进行和计划进行的研究项目框架中具有核心重要性，这些项目旨在阐明高度可调离子液体与复杂生物分子（如肽和小蛋白质）之间的结构相互作用关系，以及研究酶促反应的动力学。为此，溶液和更具挑战性的高粘度样品的高分辨率多维NMR实验是必要的。随着建立高分辨率魔角旋转NMR工具箱，这样的测量是可能的，在该集团。在计划的研究项目中，这种NMR技术将进一步应用于监测各种细长结构的肽和小蛋白质溶解在离子液体中时的构象变化和局部结构波动，以及探测离子液体对高度稳定的结构生物分子的影响。除了结构特征，NMR光谱将用于研究不同离子液体的动力学，以及通过利用其弛豫特性溶解在其中的生物分子。准确表征离子液体与生物催化剂或肽和蛋白质靶标之间的相互作用将有助于设计应用特定的离子液体。最后，NMR光谱将被应用于解决切割的肽酶靶向各种抗菌肽的偏好和动力学参数，这样的酶促反应将被导出。此外，400 MHz NMR光谱仪将用于MLU Halle的常规分析测量。