Herpesviridae infection in Okinawa : With special reference to infection to nervous system

单纯疱疹病毒：从冲绳地区生殖器疱疹中分离到2型疱疹病毒（HSV2）株（10株），其中K1、K2、K3株和实验室标准株SAV分别腹腔接种BALB/cN和C57BL/6N小鼠。C57BL/6M小鼠明显可见脊髓炎。死亡前24小时，C57BL/6N小鼠出现尿失禁和直肠失禁。在死亡前24小时内，小鼠出现尿失禁、直肠失禁和后腿截瘫。脊髓出现随机分布的严重坏死，主要位于脊髓下部。在BALB/cN小鼠中，由于小鼠在接种病毒后不久死亡，临床症状未明显观察到。尽管C57BL/6N小鼠的脊髓坏死比BALB/cN小鼠更为突出，但脑坏死只在C57BL/6N小鼠中出现，而在BALB/cN小鼠中没有出现。然而，在大脑中，病毒被PCR和免疫组织化学证实。毒力最弱的毒株K3未引起脊髓坏死，无动物死亡。实验性脊髓炎既依赖于病毒株，也依赖于小鼠株。人8型疱疹病毒（卡波西肉瘤相关疱疹病毒，KSHV）：对93例良恶性血管病变进行PCR分析，其中5例为卡波西肉瘤。5例卡波西肉瘤及化脓性肉芽肿1例KSHV阳性。而其他血管病变中梭形细胞血管内皮瘤6例、卡波西型血管内皮瘤1例、血管肉瘤8例、血管瘤46例、血管球瘤7例、化脓性肉芽肿19例均阴性。对冲绳产KSHV的序列分析表明，该病毒属于c型EBV，并对其在口腔鳞状细胞癌中的表达进行PCR和原位杂交分析。EBV DNA主要表现在浸润淋巴细胞上。

Herpes simplex virus : Herpessimplex virus type2 (HSV2) strains (10 strains) were isolated from genital herpes in Okinawa Among them, K1, K2 and K3 strains and standard laboratory strain, SAV,were inoculated intraperitoneally into BALB/cN and C57BL/6N mice. The myelitis was clearly observed in C57BL/6M mice. Within 24 hours before death, the mice showed urinary and rectal incontinence observed in C57BL/6N mice. within 24 hours before death, the mice showed urinary and rectal incontinence and paraplegia of the hind legs. Randomly distributed, severe necrosis was demonstrated in the spinal cord, mainly at the lower cord. In BALB/cN mice the clinical symptoms were not clearly observed, as the mice died shortly after viral inoculation. Although spinal cord necrosis was more prominent in C57BL/6N mice than BALB/cN mice, brain necrosis was only found later, and not in former. In the brain, however, the virus was demonstrated by both PCR and immunohistochemistry. The weakest virulent strain K3, did not cause spinal cord necrosis, and no animals died. Experimental myelitis depended on both viral strain and also mouse strain.Human herpesvirus type 8 (Kaposi sarcoma associated herpesvirus, KSHV) : PCR analysis of 93 cases of benign and malignant vascular lesions including 5 cases of Kaposi sarcoma was performed. All 5 cases of Kaposi sarcoma and one of granuloma pyogenicum were positive for KSHV.But other vascular lesions, 6 spindle cell hemangioendotheliomas, 1 kaposi form hemangioendothelioma, 8 angiosarcomas, 46 hemangiomas, 7 glomus tumors and 19 pyogenic granulomas were negative for KSHV.Sequence analysis of KSHV from Okinawa revealed that it was classified into type C.EBV : PCR and in situ hybridization analysis of EBV on oral squamous cell carcinomas were perfomed. EBV DNA was mainly demonstrated on the infiltrated lymphocytes.