The funcation of parathyroid hormone-related protein.

甲状旁腺激素相关蛋白的功能。

• 批准号: 08670231
• 负责人: MATSUSHITA Hiroshi
• 金额: $ 1.41万
• 依托单位: Okinaka Menorial Institute for Medical Researach
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670231/
• 关键词: PTHrP; Parathyriud; Immunohistochemistry; in sitsu Hybridization; mRNA; PCNA; Proliferation; PTH

The localization of parathyroid hormone(PTH)and PTH-related protein(PTHrP)were studied in 42 hyperplastic parathyroid glands in long term hemodialysis cases with immunohistochemistry and in situ hybridization. Results were compared with proliferaative activity(PCNA index : counts of proliferating cell nucleaar antaigen-positive cells/100 cells)on the same section used for PTH/PTHrP immunostaining. Ten normal glands weree studied as the control.In hyperplasia, cells positive for either PTH or PTHrP,or both, were observed immunohisstochemically. The areas expressing PTHrP mRNA were completely coincided with those positive for PTHrP immunohistochemically. Oxyphil or transitional oxyphil cells were consistently positive for PTHrP and showed a tendency for grouping together. The PCNA positive cells were rare in normal glands with index of 0.22(]SY.+-。[)0.09(mean (]SY.+-。[) SEM). Proliferating cell components were significantly increased in hyperplastic cases and indices for PTHrP positive cells were 1.25(]SY.+-。[)0.16 and tose for PTHrP negative cells were 7.8(]SY.+-。[)0.52. Thus, the proliferative activity in PTHrP positive cells was sgnificantly loweer than their PTHrP negative conterparts in secondary hyperplasia(P<0.0001).PTHrP is now mostly suspected to act as an autocrine or a paracrine substance regulating cell growth or differentiation, although it was originally reported as the hypercalcemic substance in malignancies. On the basis of suppressed cell proliferation in PTHrP positive cells, we suggest a functional role of PTHrP as growth suppresser in the human parathyroid glands.

应用免疫组织化学和原位杂交技术，对42例长期血透患者甲状旁腺增生组织中甲状旁腺激素（PTH）及其相关蛋白（PTHrP）进行了定位研究。将结果与用于PTH/PTHrP免疫染色的同一切片上的增殖活性（PCNA指数：增殖细胞核抗原阳性细胞计数/100个细胞）进行比较。10例正常腺体作为对照，增生腺体中有PTH或PTHrP阳性或两者均阳性的细胞。PTHrP mRNA表达区与化学染色阳性区完全一致。嗜酸性或过渡性嗜酸性细胞PTHrP一致阳性，并表现出聚集在一起的趋势。PCNA阳性细胞在正常腺体中较少见，其指数为0.22 ± 0.01。[）0.09（平均值（）SY. ±. [）SEM）。增生性病变中增殖细胞成分明显增多，PTHrP阳性细胞指数为1.25（± SD）。PTHrP阴性细胞的Tose为7.8 ± 0.16。[）0.52。PTHrP阳性细胞的增殖活性明显低于阴性细胞（P<0.0001）。PTHrP最初被认为是恶性肿瘤中的高钙分泌物质，但现在大多数人怀疑它是一种调节细胞生长或分化的自分泌或旁分泌物质。在PTHrP阳性细胞的细胞增殖抑制的基础上，我们建议PTHrP作为生长抑制剂在人甲状旁腺的功能作用。

项目成果

Matsushita H: "Pathology of the parathyroid glands.In Bloodworth's Endocrine Pathology,3rd Ed." Williams & Wilkins,Baltimore, 709 (1996)

Matsushita H：“甲状旁腺的病理学。见 Bloodworth 的内分泌病理学，第三版。”

Matsushita H,et al.: "Co-secretion of parathyroid hormone and parathyroid hormone-related protein via a regulated pathway in human paratahyroid adenoma cells." Am J Pathol. 150. 861-871 (1997)

Matsushita H 等人：“通过人甲状旁腺腺瘤细胞中的调节途径共同分泌甲状旁腺激素和甲状旁腺激素相关蛋白。”

Asahi Y.: "Establishment of a clonal cell line producing granulocyte colony-stimulating factor and parathyroid hormone-related protein from a lung cancer patient with leukocytosis and hypercalcemia." Jpn J Cancer Res. 87. 451-458 (1996)

Asahi Y.：“从患有白细胞增多和高钙血症的肺癌患者中建立了产生粒细胞集落刺激因子和甲状旁腺激素相关蛋白的克隆细胞系。”

Watanabe HA, Matsushita H: "Oesophageal carcinoma with humoral hypercalcaemia of malignancy and leucocytosis." Eur J Gastroenterol Hepatol. 8. 497-499 (1996)

Watanabe HA、Matsushita H：“食管癌伴恶性肿瘤体液高钙血症和白细胞增多。”

松下 央: "副甲状腺疾患の病理診断の進歩と限界" 日本内分泌学会雑誌. 73. 109 (1997)

Hiroshi Matsushita：“甲状旁腺疾病病理诊断的进展和局限性”日本内分泌学会杂志 73. 109 (1997)。