The funcation of parathyroid hormone-related protein.

应用免疫组织化学和原位杂交技术，对42例长期血液透析患者增生性甲状旁腺进行了甲状旁腺激素(PTH)和甲状旁腺相关蛋白(PTHrP)的定位研究。将结果与PTH/PTHrP免疫组织化学染色同一切片上的增殖活性(增殖细胞核抗原阳性细胞数/100个细胞)进行比较。以10例正常腺体为对照，用免疫组织化学方法观察到增生组织中甲状旁腺激素和/或甲状旁腺激素受体阳性的细胞。免疫组织化学结果显示，PTHrP mRNA表达的区域与PTHrP阳性区域完全一致。嗜酸性或过渡性嗜氧性细胞始终呈PTHrP阳性，并有聚集在一起的趋势。增殖细胞核抗原阳性细胞在正常腺体中很少见，阳性细胞指数为0.22(-)。[)0.09(平均值(]正数+-。[)扫描电子显微镜)。增生性病例中增殖细胞成分明显增多，PTHrP阳性细胞指数为1.25(sy.+-)。[]0.16，对PTHrP阴性细胞Tose为7.8(]sy.+-。[)0.52。因此，PTHrP阳性细胞的增殖活性明显低于PTHrP阴性细胞(P&lt；0.0001)。尽管PTHrP最初被认为是恶性肿瘤中的高钙物质，但目前大多认为它是一种自分泌或旁分泌物质，调节细胞的生长或分化。在PTHrP阳性细胞增殖受到抑制的基础上，我们提出了PTHrP在人甲状旁腺中作为生长抑制因子的作用。

The localization of parathyroid hormone(PTH)and PTH-related protein(PTHrP)were studied in 42 hyperplastic parathyroid glands in long term hemodialysis cases with immunohistochemistry and in situ hybridization. Results were compared with proliferaative activity(PCNA index : counts of proliferating cell nucleaar antaigen-positive cells/100 cells)on the same section used for PTH/PTHrP immunostaining. Ten normal glands weree studied as the control.In hyperplasia, cells positive for either PTH or PTHrP,or both, were observed immunohisstochemically. The areas expressing PTHrP mRNA were completely coincided with those positive for PTHrP immunohistochemically. Oxyphil or transitional oxyphil cells were consistently positive for PTHrP and showed a tendency for grouping together. The PCNA positive cells were rare in normal glands with index of 0.22(]SY.+-。[)0.09(mean (]SY.+-。[) SEM). Proliferating cell components were significantly increased in hyperplastic cases and indices for PTHrP positive cells were 1.25(]SY.+-。[)0.16 and tose for PTHrP negative cells were 7.8(]SY.+-。[)0.52. Thus, the proliferative activity in PTHrP positive cells was sgnificantly loweer than their PTHrP negative conterparts in secondary hyperplasia(P<0.0001).PTHrP is now mostly suspected to act as an autocrine or a paracrine substance regulating cell growth or differentiation, although it was originally reported as the hypercalcemic substance in malignancies. On the basis of suppressed cell proliferation in PTHrP positive cells, we suggest a functional role of PTHrP as growth suppresser in the human parathyroid glands.