Expression of interleukin-12 in synovial tissue from patients with rheumatoid arthritis.

类风湿性关节炎患者滑膜组织中白介素 12 的表达。

• 批准号: 08670518
• 负责人: YAMAMURA Masahiro
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670518/
• 关键词: interferon-gamma; interleukin-12; macrophages; osteoarthritis; rheumatoid arthritis; T cells; Th1 cells; Th1型反応

The importance of interleukin-12 as a factor in the interferon-gamma (IFN-gamma) -dominant T cell cytokine response in the synovial tissue of patients with rheumatoid arthritis (RA) was examined. Synovial tissues from RA patients more strongly expressed IL-12 p40 mRNA than did osteorthritis (OA) tissues, as determined by RT-PCR.Dissociated tissue cells from RA patients sspontaneously released detectable amounts of IL-12 p70 in culture, whereas production of IL-12 by OA tissues was limited. By immunohistochemical analysis, IL-12-producing cells were localized mainly in the sublining layr of RA synovium, and mostly expressed the CD68 antigen. Levels of IFN-gamma production by RA synovial tissue cells were potently and selectively enhanced by IL-12. The ability of IL-2-expanding synovial T cells to produce IFN-gamma was augmented by costimulation with IL-12 and diminished by anti-IL-12, while it was not affected by IL-4. These dada suggest that IL-12, produced mainly by macrophage-lineage cells, may be involved in IFN-gamma dominant cytokine production by infiltrating T cells in joints with chronic RA.

白细胞介素-12作为干扰素-γ（IFN-γ）-显性T细胞细胞因子反应的因子在类风湿性关节炎（RA）患者的滑膜组织中的重要性进行了检查。RT-PCR结果显示，RA患者滑膜组织IL-12 p40 mRNA表达较OA组织强，RA患者游离组织细胞在培养液中自发释放可检测量的IL-12 p70，而OA组织产生IL-12的能力有限。免疫组化显示IL-12阳性细胞主要分布于RA滑膜的衬里下层，且多表达CD 68抗原。RA滑膜组织细胞产生IFN-γ的水平被IL-12有效地和选择性地增强。IL-2扩增的滑膜T细胞产生IFN-γ的能力通过与IL-12共刺激而增强，通过抗IL-12而减弱，而IL-4不影响。这些数据表明，IL-12，主要由巨噬细胞系细胞产生，可能参与IFN-γ占主导地位的细胞因子的生产与慢性RA关节浸润T细胞。

项目成果

山村 昌弘: "慢性関節リウマチ滑膜におけるサイトカイン発現異常" 医学のあゆみ. 182・9. 573-578 (1997)

Masahiro Yamamura：“类风湿性关节炎滑膜中细胞因子的异常表达”医学史182・9（1997）。

Yoshitaka Morita, Masahiro Yamamura, Kenichiro Nishida,et al.: "Expression of Interleukin-12 in Synovial Tissue From Ritients with Rheumatoid Ardiricis." Arthritis & Rheumatism. 41・2. 306-314 (1998)

Yoshitaka Morita、Masahiro Yamamura、Kenichiro Nishida 等：“类风湿性关节炎和风湿病滑膜组织中白细胞介素 12 的表达”41・2。

Masahiro Yamamura: "CD8^+Th2 cells and suppressor T cells" Clin Immunol. 28 (2). 245-249 (1996)

Masahiro Yamamura：“CD8^ Th2 细胞和抑制性 T 细胞”Clin Immunol。

Masahiro Yamamura, Hirofumi Makino: "Cytokine expression in rheumatoid arthritis" Igakuno-ayumi. 183 (8). 573-578 (1997)

Masahiro Yamamura、Hirofumi Makino：“类风湿性关节炎中的细胞因子表达”Igakuno-ayumi。

Morita, Y., et al.: "Expression of interleukin-12 in synovial tissue from patients with rheumatoid arthritis" Arthritis Rheum. 41・2. 306-314 (1998)

Morita, Y., et al.：“类风湿性关节炎患者滑膜组织中白细胞介素 12 的表达”Arthritis Rheum 41・2 (1998)。