权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mechanisms for premenstrual exacerbation of asthama

经前哮喘恶化的机制

基本信息

批准号：
08670645
负责人：
OHRUI Takashi
金额：
$ 1.41万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670645/
关键词：
Bronchial asthma premenstrual asthma Progesterone estrogen Brown Norway rat antigen challenge Late airway response inflammatory cells 好酸球浸潤

项目摘要

Sixteen female virgin Brown Norway Rats were sensitized with ovalubumin in combination with Aluminium hydroxide injected subcutaneously and Bordetera Pertussis vaccine intraperitoneally. After sensitization was performed, female rats were ovariectomized and divided into four groups (n=4 each) as described previously. The first group consisted of ovariectomized rats which received estradiol at 3,4,7 and 8 days after ovariectomy, and progesterone at 4 and 8 days cyclically (mean basic cycle length is 4.1 day) after ovariectomy. The second group was treated only with estradiol at 3,4,7 and 8 days, the third group was treated only with progesterone at 4 and 8 days, while the fourth group received no steroid treatment. At 8 days, estradiol and progesterone treatment was done at 4 hours before the start of the antigen challenge. Eight days after ovariectomy, all groups were challenged with aerozolized ovalubumin and intra-airway pressures were monitored under mechanical ventilation to detect immediate and late airway responses followed by antigen exposure. Broncho-alveolar lavage (BAL) and histological examination were performed to evaluate the airway inflammation associated with antigen exposure eight hours after challenge. In the group received cyclical replacement of estrogen and progesterone, the magnitude of both immediate and late airway responses was significantly lowered compared to the other groups, and the number of neutrophils recovered in the BAL fluid eitht hours after challenge was significantly lowered compared to other groups. We conclude that cyclical administration of progesterone and estrogen inhibits the early-and late- phase responses to inhaled allergen in female sensitized Brown Norway Rat.

用卵清蛋白与皮下注射的氢氧化铝和腹膜内注射的百日咳博德特氏杆菌疫苗联合致敏16只雌性未交配过的Brown Norway大鼠。进行致敏后，切除雌性大鼠卵巢，并如前所述分成4组（每组n=4）。第一组为去卵巢大鼠，分别于去卵巢后3、4、7、8d给予雌二醇，于去卵巢后4、8d给予孕酮（平均基础周期为4.1d）。第2组在第3、4、7、8天仅给予雌二醇治疗，第3组在第4、8天仅给予孕酮治疗，第4组不给予激素治疗。在第8天，在抗原攻击开始前4小时进行雌二醇和孕酮处理。卵巢切除术后8天，所有组均用雾化卵蛋白激发，并在机械通气下监测气道内压，以检测抗原暴露后的即刻和晚期气道反应。激发后8 h行支气管肺泡灌洗（BAL）和组织学检查，以评估抗原暴露相关的气道炎症。在接受雌激素和孕激素周期性替代治疗的组中，与其他组相比，即刻和晚期气道反应的幅度均显著降低，并且与其他组相比，激发后8小时在BAL液中恢复的中性粒细胞数量显著降低。我们的结论是，周期性管理孕激素和雌激素抑制早期和晚期阶段的反应，吸入过敏原在女性致敏布朗挪威大鼠。