Intrapulmonary Distribution of Idiopathic and Collagen Vascular Disease associated Interstitial Pneumonia, and Local Expression of Cytokine mRNA.

特发性和胶原血管疾病相关间质性肺炎的肺内分布以及细胞因子 mRNA 的局部表达。

• 批准号: 08670658
• 负责人: SUZUKI Eiichi
• 金额: $ 1.34万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670658/
• 关键词: idiopathic interstitial pneumonia; collagen vascular disease associated interstitial pneumonia; intrapulmonary distribution; image analyzer; broncho-alveolar lavage; intrapulmonary cells; cell surface marker; pulmonary fibroblast; 線維芽細胞

The objects of this project was to clarify the difference of intrapulmonary distribution and local expression of cytokine mRNA between idiopathic interstitial pneumonia (HP) and interstitial pneumonia with collagen vascular disease (IP-CVD). There were the characteristic findings of intrapulmonary lesions of HP and IP-CVD by evaluation of chest high-resolution computed tomography and image analyzer. There was the difference in cell surface markers of lymphocyte and alveolar macrophage in broncho-alveolar lavage fluid (BALE) by FACScan between IIP and IP-CVD.And there was the difference in cytokine expression of alveolar macrophage in BALF by competitive PCR method. In this project, procollagen type I carboxyterminal propeptide levels in supernatant fluid of pulmonary fibroblast culture and serum were measured. From these results, I hope to become clear something of pathogenesis in lip and IP-CVD.

本课题旨在探讨特发性间质性肺炎（HP）和间质性肺炎伴胶原血管病（IP-CVD）肺内细胞因子mRNA分布和表达的差异。胸部高分辨率CT及图像分析仪对HP及IP-CVD的肺内病变均有特征性表现。FACScan检测结果显示，IIP组和IP-CVD组BALE中淋巴细胞和肺泡巨噬细胞的细胞表面标志物存在差异，竞争PCR检测结果显示，BALF中肺泡巨噬细胞的细胞因子表达存在差异。本研究测定了肺成纤维细胞培养上清液和血清中Ⅰ型前胶原羧基末端前肽的水平。通过这些结果，我希望能对唇和IP-CVD的发病机制有所了解。

项目成果

Toshinori Takada,et al.: "Clinical Features of Polymyositis/Dermatomyositis with Steroid-Rosistent Interstitial Lung Disease." Infernal Medicine. 37. 669-673 (1998)

Toshinori Takada 等人：“多发性肌炎/皮肌炎伴类固醇性间质性肺病的临床特征”。

Hiroyo Kourakata, et al.: "Flow cytometric Analysis of Broncho-Alveclar Large Fluid cells in Polymyositis/Dermatomyositis with Interstitial Pneumonia." Respirology. (in press).

Hiroyo Kourakata 等人：“多发性肌炎/皮肌炎伴间质性肺炎中支气管肺泡大液细胞的流式细胞术分析”。

Masaaki Nakano, et al.: "Determination of procollagen type I carboxyterminal propeptide in Japanese patients with systemic sclerosis" Japanese Journal of Rheumatology. 8. 147-155 (1998)

Masaaki Nakano 等人：“日本系统性硬化症患者 I 型前胶原羧基末端前肽的测定”日本风湿病杂志。

Toshinori Takada, et al.: "Expression of specific mRNA of peripheral blood mononuclear cell in patients with diffuse panbronchiolitis by cDNA repesentational difference analysis (RDA) method" The Japanese Journal of Antibiotics. 50. 130-132 (1997)

Toshinori Takada 等人：“通过 cDNA 重复差异分析 (RDA) 方法表达弥漫性全细支气管炎患者外周血单核细胞的特异性 mRNA”，日本抗生素杂志。

Masaaki Nakano, et al.: "Determination of procollagen type I carboxyterminal propeptide in Japanese patients with systemic sclerosis." Japanese Journal of Rheumatology. 8. 147-155 (1998)

Masaaki Nakano 等人：“日本系统性硬化症患者 I 型前胶原羧基端前肽的测定。”