Intrapulmonary Distribution of Idiopathic and Collagen Vascular Disease associated Interstitial Pneumonia, and Local Expression of Cytokine mRNA.

目的探讨特发性间质性肺炎（HP）与间质性肺炎合并胶原血管病（IP-CVD）肺内分布及局部细胞因子mRNA表达的差异。通过胸部高分辨率计算机断层扫描和图像分析仪的评估，发现HP和IP-CVD肺内病变的特征性表现。支气管肺泡灌洗液（BALE）中淋巴细胞和肺泡巨噬细胞的细胞表面标记物在IIP和IP-CVD之间存在差异。通过竞争性PCR法检测BALF中肺泡巨噬细胞细胞因子表达的差异。本项目测定肺成纤维细胞培养上清液和血清中I型前胶原羧基末端前肽水平。通过这些结果，我希望对唇部和IP-CVD的发病机制有所了解。

The objects of this project was to clarify the difference of intrapulmonary distribution and local expression of cytokine mRNA between idiopathic interstitial pneumonia (HP) and interstitial pneumonia with collagen vascular disease (IP-CVD). There were the characteristic findings of intrapulmonary lesions of HP and IP-CVD by evaluation of chest high-resolution computed tomography and image analyzer. There was the difference in cell surface markers of lymphocyte and alveolar macrophage in broncho-alveolar lavage fluid (BALE) by FACScan between IIP and IP-CVD.And there was the difference in cytokine expression of alveolar macrophage in BALF by competitive PCR method. In this project, procollagen type I carboxyterminal propeptide levels in supernatant fluid of pulmonary fibroblast culture and serum were measured. From these results, I hope to become clear something of pathogenesis in lip and IP-CVD.