Molecular biological changes of ADP/ATP carrier protein in cardiomyopathic hamsters

心肌病仓鼠ADP/ATP载体蛋白的分子生物学变化

• 批准号: 08670827
• 负责人: HAYASHI Yuusaku
• 金额: $ 0.7万
• 依托单位: The Jikei University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670827/
• 关键词: ATP; ADP carrier protein; mitochondrial membrane; cardiomyopathy; hypertrophy; diabetic hearts; adaptation; 肥大心; ノーザンブロット; 拡張形心筋症; ミトコンドリア遺伝子; 圧負荷肥大心; ミトコンドリア

The ATP/ADP carrier protein (AAC) is an integral protein present in the inner mitochondrial membrane that performs the exchange of cytoplasmic and intramitochondrial ADP and ATP.The ACC content of the myocardium was studied in J-2-N cardiomyopathic hamster, renovascular hypertensive rats and diabetic rats.The content of ACC protein was significantly decreased in cardiomyopathic hamster. On the other hand, ACC m-RNA level was increased in cardiomyopathic hamster. In renovascular hypertensive rat (2K-1C), left ventricular hypertrophy and abnormal changes of myosin isoenzyme pattern were observed 8 weeks after an operation. The content of ACC protein was significantly decreased in operated rats. In streptozotocin-induced diabetic rats, the content of ACC protein was significantly increased.These results suggest that the changes of the AAC protein play an important role in the pathogenesis of hemodynamic alteration of cardiomyopathy, hypertrophy and diabetic hearts. Moreover, these changes were considered to be an adaptation which contribute to maintain efficient force development with low oxygen and energy utilization in several cardiac deceases.

ATP/ADP载体蛋白(AAC)是存在于线粒体内膜中的整合蛋白，执行细胞质和线粒体内ADP和ATP的交换。在J-2-N心肌病仓鼠、肾血管性高血压大鼠和糖尿病大鼠中研究了心肌的ACC含量。心肌病中ACC蛋白的含量显着降低 仓鼠。另一方面，心肌病仓鼠的 ACC mRNA 水平升高。肾血管性高血压大鼠（2K-1C）术后8周观察到左心室肥厚和肌球蛋白同工酶模式异常变化。手术大鼠ACC蛋白含量显着降低。链脲佐菌素诱导的糖尿病大鼠中ACC蛋白含量显着升高。这些结果提示AAC蛋白的变化在心肌病、肥厚心脏和糖尿病心脏的血流动力学改变的发病机制中起重要作用。此外，这些变化被认为是一种适应，有助于在几种心脏疾病中保持低氧和能量利用的有效力量发展。

项目成果

加藤光敏他: "続心臓代謝実験法 「ADP/ATP carrier protein測定法」" 株式会社六法出版, 541-544 (1998)

加藤光俊等：“继续心脏代谢实验方法“ADP/ATP载体蛋白测定方法”，六方出版株式会社，541-544(1998)

Li-Mei Liu et al.: "The alterrations of myosin isoenzymes and ADP/ATP carrier (AAC) in Goldblatt hypertensive rats" J.Mol.Cell.Cardiol.28. A336 (1996)

Li-Mei Liu 等人：“Goldblatt 高血压大鼠中肌球蛋白同工酶和 ADP/ATP 载体 (AAC) 的改变”J.Mol.Cell.Cardiol.28。