STUDY ON PATHOGENECITY OF GROUP A STREPTOCOCCUS BY GENOME ANALYSES : RELEVANCE TO STREPTOCOCCAL TOXIC SHOCK SYNDROME

A群链球菌的基因组致病性研究：与链球菌中毒性休克综合征的相关性

• 批准号: 08670845
• 负责人: MURONO Koichi
• 金额: $ 1.47万
• 依托单位: ASAHIKAWA MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670845/
• 关键词: GROUP A STREPTOCOCCUS; M type 1; INVASIVE DISEASE; TOXIC SHOCK SYNDROME; GENOME; PATHOGENESIS; PULSED-FIELD GEL ELECTROPHORESIS; 血清型T1

Background. In Japan invasive group A streptococcal infections such as sepsis and toxic shock syndrome (TSS) have increased since 1992. As is the case in the United States and Europe, M1 serotype is predominant among the isolates from Japanese patients.Methods. By restriction enzyme digestion and pulsed-field gel electrophoresis, we investigated the whole genomic DNA profiles of 95 M type 1 group A streptococcal strains isolated from patients with serious diseases including sepsis, TSS, necrotizing fasciitis and nonsuppurative complications and with uncomplicated pharyngitis during 1979 - 1996 in Japan.Results. The genome profiles among 8 of 10 isolates from patients with serious diseases in 1979 - 1991 were all the same and were shared by the profiles of the 35 of 48 isolates from patients with uncomplicated pharyngitis in 1982 -1991. All 18 strains isolated from patients with invasive diseases in 1992 - 1996 had a unique profile, which was shared by the profiles of 18 of 19 isolates from uncomplicated pharyngitis during the same period. This genomic profile was distinct from the predominant or any other profiles before 1992, and it was found to be a new clone.Conclusions. The emergence and spread of this new clone of M type 1 streptococcus after 1991 may be associated with the increase in invasive streptococcal infections that occurred during the same period in Japan. Genomic profiles as well as serotypes of streptococcal isolates are important for the epidemiology of clinical relevance in streptococcal diseases.

背景在日本，自1992年以来，侵袭性A组链球菌感染，如败血症和中毒性休克综合征（TSS）有所增加。与美国和欧洲的情况一样，M1血清型在来自日本患者的分离株中占主导地位。采用限制性内切酶消化和脉冲场凝胶电泳技术，对1979 ~ 1996年日本95株M 1型A组链球菌进行全基因组DNA图谱分析。1979 ~ 1991年10株重症患者分离株中有8株的基因组图谱完全相同，而1982 ~ 1991年48株无并发症咽炎患者分离株中有35株的基因组图谱相同。1992 - 1996年从侵袭性疾病患者中分离出的所有18种菌株都具有独特的特征，同一时期从无并发症的咽炎中分离出的19种菌株中有18种菌株也具有相同的特征。这一基因组图谱与1992年以前的主要图谱或任何其他图谱都不同，并且被发现是一个新的克隆。1991年后这种新的M 1型链球菌克隆的出现和传播可能与日本同期发生的侵袭性链球菌感染的增加有关。链球菌分离株的基因组谱和血清型对于链球菌疾病的临床相关性的流行病学是重要的。

项目成果

平野至規、室野晃一、西條政幸、藤田晃三、ほか: "T1型A群溶連菌のゲノムプロファイルの変化について" 小児感染免疫. 9・2. 185- (1997)

Toshiki Hirano、Koichi Murono、Masayuki Saijo、Kozo Fujita 等：“T1 型链球菌基因组谱的变化”小儿感染免疫学 9・2。

平野至規: "T1型A群溶連菌のゲノムプロファイルの変化について" 小児感染免疫. 9・2. 185- (1997)

Toshiki Hirano：“T1 型 A 型链球菌基因组谱的变化”《儿科感染免疫学》9·2。

室野晃一、西條政幸、藤田晃三、村井貞子: "非化膿性合併症、重症感染症患者から分離されたA群溶連菌のゲノム解析" 小児感染免疫. 8・2. 138- (1996)

Koichi Murono、Masayuki Saijo、Kozo Fujita、Sadako Murai：“从非化脓性并发症和严重感染患者中分离出的 A 族链球菌的基因组分析”《儿科感染与免疫学》8・2。

Koichi Murono, Kozo Fujita, Masayuki Saijo, Yoshiki Hirano, Jiwei Zhang, Teiko Murai.: "Emergence and spread of a new clone of M type 1 group A Streptococcus coincident with the increase in invasive diseases in Japan." Pediatr Infect Dis J. 18(3). 254-257

Koichi Murono、Kozo Fujita、Masayuki Saijo、Yoshiki Hirano、Jiwei Zhu、Teiko Murai.：“M 1 型 A 组链球菌新克隆的出现和传播与日本侵袭性疾病的增加同时发生。”

室野晃一、西條政幸、Jiwei Zhang、藤田晃三、ほか: "臨床分離A郡溶連菌のゲノムプロファイルについて" 感染症学雑誌. 70・8. 903- (1996)

Koichi Murono、Masayuki Saijo、Jiwei Zhang、Kozo Fujita 等人：“临床分离的 A 族链球菌的基因组谱”《传染病杂志》70, 8. 903- (1996)。