A study of 5-FU metabolism in fatty liver and hepatocellular carcinoma induced by choline deficient diet, using 19F-MRS.

目的：用~(19)F-MRS和~(31)P-MRS分别观察5-氟尿嘧啶代谢(合成成氟核苷酸(Fnct)和分解成α-氟-β-丙氨酸(FBAL))和果糖代谢(主要是转化为果糖-1-磷酸)来评价脂肪肝。材料和方法：雄性Wistar SLC大鼠在BEM170/200(4.7T，Otsuka Electronics)上用直径17 mm的表面线圈进行检查。以胆碱缺乏饮食(镉饮食)2周或4周诱导大鼠脂肪肝。静脉注射5-FU后100分钟获得~(19)F-MR波谱。测定注射果糖前后的31P-MR波谱。结果：在19F-MRS中，镉饮食组的Fnct峰明显升高，FBAL峰受到抑制。在~(31)P-MRS中，镉饮食组大鼠血清中磷酸单酯(PME)和三磷酸腺苷(ATP)的时间进程与对照组无显著差异。结论：我们检测到反映肝损伤恢复反应的Fnct峰值，但果糖代谢的差异不明显。在受损的肝脏中，受损的代谢途径和严重程度因疾病和个体而异。在体磁共振波谱的动态研究有可能检测到受损的部位和严重程度。我们认为，尝试一些不同的方法来评估肝功能是非常重要的，体内动态MRS可能是有价值的。

Purpose : To assess fatty liver by 19F-MRS and 31P-MRS,observing 5-fluorouracil metabolism (anabolize to fluoronucleotide (Fnct) and catabolize to alpha-fluoro-beta-alanine (FBAL)) and fructose metabolism (mainly inverted to fructose-1-phosphate), respectively.Materials and Methods : Male Wistar Slc rats were examined on BEM170/200 (4.7T,Otsuka Electronics) with 17mm-diameter surface coil. Fatty liver was induced by choline deficient diet (CD diet) given for 2 or 4 weeks. 19F-MR spectra were obtained for 100min.after 5-FU intravenous injection. 31P-MR spectra were obtained pre and post fructose injection. 19F-MRS and 31P-MRS studies were performed in separated experiments.Results : In 19F-MRS,high Fnct peak and suppressed FBAL peak was observed in CD diet group. In 31P-MRS,no significant difference could be seen between CD diet group and control diet group about the time course of phosphomonoester (PME) and adenosine-triphosphate (ATP).Conclusion : We detected the high peak of Fnct that may reflect the recovering reaction from liver damage but the difference of fructose metabolism was not evident. In damaged liver, the injured metabolic pathway and severity is different among the diseases and individuals. The dynamic study with in vivo MRS has the possibility to detect the impaired point and the severity. We proposed that it is very important to try some different methods to evaluate liver function and in vivo dynamic MRS may be valuable for it.