Cell dynamics from the viewpoint of cell proliferation and cell death in several hereditary intestinal polyposis syndrome.

从细胞增殖和细胞死亡的角度来看几种遗传性肠息肉病综合征的细胞动力学。

• 批准号: 08671417
• 负责人: IWAMA Takeo
• 金额: $ 1.28万
• 依托单位: Sasaki Institute (1997)Tokyo Medical and Dental University (1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671417/
• 关键词: Familial adenomatous polyposis; Juvenile polyposis; Peutz-Jeghers syndrome; Cell proliferation; Apoptosis; アポートシス; 大腸癌; 遺伝性消化管腫瘍

The patients included 15 adenomas in FAP(familial adenomatous polyposis), 3 Juvenile polyposis, 5 Peutz-Jeghers syndrome, 14 sporadic adenomas, 46 sporadic colorectal carcinomas and 20 normal colorectal epithelium. The specimen were processed for TUNEL(terminal uridine deoxyuncleotide nick end labeling)and PCNA(proliferating cell nuclear anatigen)staining. Adenomas showed high PCNA and TUNEL indices compared to the normal epithelium. There were no differences in TUNEL and PCNA indices between adenomas of FAP and sporadic adenomas. Juvenile polyposis showed no TUNEL and PCNA positive cells. Peutz-Jeghers syndrome showed high PCNA indices and normal TUNEL indices. Carcinoma showed higher PCNA and TUNEL indidces was useful in refleclting difference of cell proliferation between differnt hereditary conditions.

其中家族性腺瘤性息肉病15例，幼年型息肉病3例，Peutz-Jeghers综合征5例，散发性腺瘤14例，散发性结直肠癌46例，正常结直肠上皮20例。标本行TUNEL（末端尿苷脱氧核苷酸缺口末端标记）和PCNA（增殖细胞核抗原）染色。与正常上皮相比，腺瘤显示出高PCNA和TUNEL指数。FAP腺瘤与散发性腺瘤的TUNEL和PCNA指数无差异。幼年型息肉病未见TUNEL和PCNA阳性细胞。Peutz-Jeghers综合征表现为高PCNA指数和正常TUNEL指数。癌组织中PCNA表达增高，TUNEL标记可反映不同遗传状态下细胞增殖的差异。

项目成果

Yange Zang, Takeo Iwama, et al.: "Histochemical study of poptosis and cell proliferation in hereditary intestinal diseases." Journal of Medical and Dental Sciences. 45(2)(in printing). (1997)

Yange Zang、Takeo Iwama 等人：“遗传性肠道疾病中细胞凋亡和细胞增殖的组织化学研究。”

張燕華,岩間毅夫: "大腸癌におけるアポートシス発現の検討" 日本大腸肛門病学会雑誌. 49. 807- (1996)

张艳华，Takeo Iwama：“结直肠癌中细胞凋亡表达的检查”日本结肠直肠学会杂志 49. 807-（1996）。

Yange Zang, Takeo Iwama, et al.: "Cell dynamics from the viewpoint of cell proliferation and cell death in sefveral hereditary intestinal polyposis syndrome." Jpn J Gastroent Surg. 50(6). 1446 (1997)

Yange Zang、Takeo Iwama 等人：“从几种遗传性肠息肉病综合征的细胞增殖和细胞死亡的角度来看细胞动力学。”

Yange Zang, Takeo Iwama et al: "Histochemical study of opoptosis and cell proliferation in hereditory intestinol diseases." Journal of Medical and Pental Sciences. 45(2)(in printing). (1998)

Yange Zang、Takeo Iwama 等人：“遗传性肠病中细胞凋亡和细胞增殖的组织化学研究。”

張燕革, 岩間毅夫,他: "遺伝性消化管腫瘍性疾患における腫瘍の増殖と細胞死からみた増殖動態の解析" 日本消化器外科学会雑誌. 50(6). 1446 (1997)

张彦杰，Takeo Iwama等：“从遗传性胃肠道肿瘤疾病的肿瘤生长和细胞死亡的角度分析生长动力学”日本胃肠外科学会杂志50(6) 1446(1997)。