Evaluation of the acetaminophen absorption test for the early detection of small bowel transplan

对乙酰氨基酚吸收试验对小肠移植早期检测的评价

• 批准号: 08671450
• 负责人: MIYAKE Hidenori
• 金额: $ 1.41万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671450/
• 关键词: small bowel transplantation; acute rejction; acetaminophen; 吸収試験; FK506; 早期診断法

PurposeWe evaluated the efficiency of acetaminophen (AC) absorption test as a marker of graft rejection in rats orthotopic small bowel transplantation (SBTX).Materials and MethodsBN rats were used as small bowel graft's donors and Lewis rats as recipients. Orthotopic allogenic SBTX (BN-to-Lewis) was accomplised using a cuff technique. In brief, 20 cm proximal jcjunum of BN donors was exteriorized as the transplant graft. The donor SMA and portal vein was anastmosed to the recipient's left renal artery and vein. The entire small bowel was resected, and a 20 cm small bowel graft was anastomosed end-to-end with the duodenum and the remaining terminal ileum. All experimental animals were divided into five experimental groups, as follows. A (n=9) : An untrated control. B (n=15) : Extended resection of Lewis small bowel remaining 20 cm of proximal jejunum and 2 cm of terminal ileum. C (n=12) : syngenic SBTX (Lewis-to-Lewis) without immunosuppressants. D (n=15) : allogenic SBTX with FK506 (0.1mg/kg/day). E (n=15) : allogenic SBTX with FK506 (0.5mg/kg/day). Serum AC levels were measured 15 minutes following instillation of 0.15 g/kg of AC through a gastric tube 1,3,7 postoperative day (POD) in each group. The jejunal graft removed from each animal were evaluated histologically.ResultsThe graft in group D showed the progressive acute rejection histologically from 1 POD (normal or mild) to 3 (moderate) and 7 (severe) POD.Serum AC levels in group D showed significant decrease from day 1 (53.1 (]SY.+-。[) 3.9mug/ml) to day 3 (35.0 (]SY.+-。[) 12.0) and day 7 (10.9 (]SY.+-。[) 5.6). No remarkable change of serum AC levels and histological findings was observed in the other four experimental groups.ConclusionThe good correlation between serum AC levels and histological changes were observed in rats that resulted in acute rejection. The AC absorption test appears to be useful for early detection of SBTX graft rejection.

目的评价对乙酰氨基酚（AC）吸收试验作为大鼠原位小肠移植（SBTX）排斥反应指标的有效性。材料与方法以bn大鼠为小肠移植供体，Lewis大鼠为受体。采用袖带技术完成原位同种异体SBTX （BN-to-Lewis）。简而言之，将BN供体近端关节处20 cm作为移植移植物外化。供体SMA和门静脉与受体左肾动、静脉吻合。切除整个小肠，将20厘米的小肠移植物与十二指肠和剩余的回肠末端端对端吻合。所有实验动物分为5个实验组，如下所示。A (n=9)：未加对照。B (n=15): Lewis小肠扩大切除术，空肠近端剩余20cm，回肠末端剩余2cm。C (n=12)：不含免疫抑制剂的syngenic SBTX （Lewis-to-Lewis）。D (n=15)：同种异体SBTX与FK506 （0.1mg/kg/天）。E (n=15)：同种异体SBTX与FK506 （0.5mg/kg/天）。各组术后1、3、7天（POD）经胃管灌注0.15 g/kg AC 15分钟后测定血清AC水平。对每只动物的空肠移植物进行组织学评价。结果D组移植体在组织学上表现为从1个POD（正常或轻度）到3个POD（中度）和7个POD（重度）的进行性急性排斥反应。D组大鼠血清AC水平自第1天起显著降低（53.1 (p < 0.05)）。[) 3.9杯/毫升)至第3天(35.0（]）。[) 12.0]和第7天（10.9）[](5.6))。其余4个实验组血清AC水平及组织学检查结果无明显变化。结论急性排斥反应大鼠血清AC水平与组织学变化有良好的相关性。AC吸收试验似乎对SBTX移植排斥反应的早期检测有用。

项目成果

T.Miyauchi, M.Ishikawa et al: "Effect of donor-specific splenocytes via portal vein and FK506 in rat small bowel transplantation" Transplantation. 65. 27-32 (1998)

T.Miyauchi、M.Ishikawa 等人：“通过门静脉和 FK506 供体特异性脾细胞对大鼠小肠移植的影响” 移植。

T Miyauchi et al.: "Acetaminophen absorption test asa marker of small bowel transplant rejection" Transplantation. 63. 1179-1182 (1997)

T Miyauchi 等人：“对乙酰氨基酚吸收试验作为小肠移植排斥反应的标志” 移植。

T Miyauchi et al: "Acetaminophen absorption test asa marker of small bowel transplant rejection" Transplantation. 63. 1179-1182 (1997)

T Miyauchi 等人：“对乙酰氨基酚吸收试验作为小肠移植排斥反应的标志” 移植。

T.Miyauchi, M.Ishikawa et al: "Acetaminophen absorption test as a marker of small bowel transplant rejection" Transplantation. 63. 1179-1182 (1997)

T.Miyauchi、M.Ishikawa 等人：“对乙酰氨基酚吸收试验作为小肠移植排斥反应的标志” 移植。