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Role of proliferation and apoptosis in superficial tumor in the colon

增殖和凋亡在结肠浅表肿瘤中的作用

基本信息

批准号：
08671481
负责人：
KASHIWAGI Hiroshi
金额：
$ 1.34万
依托单位：
JICHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671481/
关键词：
superficial lesion flat lesion apoptosis microsatellite instability DNA mismatch repair gene cyclin D cyclin E P21 P27 P53 apopt

项目摘要

In order to determine the genetic difference between superficial lesion and polypoid lesion, proliferation and apoptosis status and microsatellite instability status were examined using colorectal lesions with differing morphology. Proliferation and apoptosis status were examined in 37 colorectal adenomas with differing morphology by immunohistochmistry using anti-bc12 and anti-Ki67 antibodies. Materials were consisted of 11 polypoid adenomas, 16 flat elevated adenomas and 10 superficial adenomas, Proliferation status was similar among polypoid adenomas, flat elevated adenomas and superficial adenomas. However, concerning the relation between apoptosis status and morphology of the adenomas, superficial adenomas were tended to carry more active apoptosis status than polypoid adenomas.Microsatellite instability(MSI) was initially reported to be found in hereditary non polyposis colorectal cancer. Recently MSI is considered to be a marker to reflect DNA mismatch repair status and may refl … More ect the biological behavior. MSI were examined in 103 sporadic colorectal cancer in advanced stages. In this series, MSI was detected in 15.7% of sporadic colorectal cancers. Positive rate of MSI in colorectal cancers in the proximal colon and in the distal colon were 33% and 6%, respectively. MSI was found more in the proximal colon.Forty-four early colorectal cancers without family history of colorectal cancers were assayed for MSI status. Materials were consisted of 28 polypoid and 16 superficial lesions, and 19 lesions cited in the proximal colon and 25 lesions in the distal colon. MSI was positive in 11.4% of early colorectal cancers. In the proximal colon, superficial adenomas showed positive MSI more frequently than polypoid adenomas(44% vs 0%, p0.O4), which may suggest the different carcinogenesis between superficial lesions and polypoid lesions.The result in the present studies suggested that the carcinogenesis was different between in the proximal colon cancer and in the distal colorectal cancer. The tumorigenesis was also considered to be different between in the superficial lesions and in the polypoid lesions in the proximal colon by means of proliferation-apoptosis status and MSI status. Less

为了确定浅表病变和息肉样病变之间的遗传差异，对不同形态的结直肠病变进行增殖和凋亡状态以及微卫星不稳定性状态的检测。应用抗bc 12和抗Ki 67抗体，通过免疫组化法检测37例不同形态结直肠腺瘤的增殖和凋亡状态。其中息肉样腺瘤11例，扁平隆起腺瘤16例，浅表腺瘤10例。然而，关于腺瘤形态与凋亡状态的关系，浅表腺瘤比息肉样腺瘤更倾向于携带更活跃的凋亡状态。微卫星不稳定性（MSI）最初报道见于遗传性非息肉病性大肠癌。近年来，MSI被认为是反映DNA错配修复状态的一个标志物，可能反映了DNA错配修复过程中的细胞凋亡。 ...更多信息观察生物学行为。对103例散发性晚期大肠癌进行MSI检测。在这个系列中，MSI在15.7%的散发性结直肠癌中被检测到。近端结肠癌MSI阳性率为33%，远端结肠癌MSI阳性率为6%。对44例无大肠癌家族史的早期大肠癌患者进行MSI检测。材料包括息肉样病变28个，浅表病变16个，近端结肠病变19个，远端结肠病变25个。MSI阳性率为11.4%。近端结肠浅表腺瘤MSI阳性率为44%，息肉样腺瘤MSI阳性率为0%（P <0.04），提示浅表性病变与息肉样病变的癌变机制不同，近端结肠癌与远端大肠癌的癌变机制不同。通过增殖-凋亡状态和MSI状态，认为近端结肠浅表病变和息肉样病变的肿瘤发生也不同。少