CELL ADHESION AND LUNG PRESERVATION

细胞粘附和肺保护

• 批准号: 08671524
• 负责人: FUKUSE Tatsuo
• 金额: $ 1.34万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671524/
• 关键词: lung transplantation.; preservation; ex vivo model; ET-Kyoto solution; dibutyryl cyclic AMP; cell adhesion; 肺移植; ウィスコンシン大学液; new ET-Kyoto液; ユーロコリンズ液

We study the effects of new ET-K solution in lung preservation, and compare it with EC and UW solutions using an ex vivo rat reperfusion model. The perfusion circuit was initiated by 30 ml of homologous blood. By means of double head roller pump, the blood passed from the venous blood reservoir through the pulmonary artery to be perfused in the examined lung. The lung effluent was returned at the same flow rate to the deoxygenator fresh lung. In fresh group, lung was flushed with saline, and then it was reperfused immediately. In the new ET-K group, UW group and EC group, lung was flushed with the new ET-K,UW,and EC,respectively. The shunt fraction, pulmonary arterial pressure, and peak inspiratory pressure in the new ET-K and UW groups were significantly better than those in the EC group, but were almost equal to those in the fresh group. In addition, this ex vivo rat reperfusion model is simple and highly reliable, and can be widely used in the studies of pulmonary preservation.We examined the effect of db-cAMP against lung injury caused by cold preservation and ischemia-reperfusion. In the fresh group, the lung was flushed with the preservative solution and reperfusion was performed immediately. In the control group and the db-cAMP group, the lung was flushed with either the solution or with a combination of the solution plus db-cAMP,respectively, and preserved at 4ﾟC at 15 hours. The shunt ratios of the reperfused lung in the db-cAMP group were as low as those in the fresh group and significantly lower than those in the control group (p<0.01). Electron microscopic examination showed less damage in te pulmonary arterial endothelium in the db-cAMP group. Immunohistochemical study using anti-rat granulocyte antibody showed less granulocytes in the db-cAMP group. We conclude that db-cAMP attenuates the lung injury by cold preservation and ischemia-reperfuion, at least partly by protection of the vascular endothelium.

我们研究了新的ET-K溶液在肺保存中的作用，并使用离体大鼠再灌注模型将其与EC和UW溶液进行比较。灌注回路由30 ml同源血液启动。采用双头滚轮泵将血液从静脉储血器经肺动脉灌注到受检肺内。肺流出物以相同的流速返回到脱氧器新鲜肺。新鲜组用生理盐水冲洗肺，然后立即再灌注。新ET-K组、UW组和EC组分别用新ET-K、UW和EC灌洗肺。新ET-K和UW组的分流分数、肺动脉压和吸气峰压明显优于EC组，但与新鲜组几乎相等。此外，该离体大鼠肺再灌注模型操作简便，可靠性高，可广泛应用于肺保存的研究。新鲜组用保存液冲洗肺，立即进行再灌注。在对照组和db-cAMP组中，分别用溶液或溶液加db-cAMP的组合冲洗肺，并在4 ℃下保存15小时。db-cAMP组再灌注肺分流率与新鲜组相当，但明显低于对照组（P<0.01）。电镜检查显示db-cAMP组肺动脉内皮损伤较轻。使用抗大鼠粒细胞抗体的免疫组织化学研究显示db-cAMP组中粒细胞较少。我们得出结论，db-cAMP减轻肺损伤的冷保存和缺血再灌注，至少部分通过保护血管内皮。

项目成果

Nakamura T, Hirata T, Fukuse T et al.: "Dibutyryl c AMP attenuates lung injury caused by cold preservation and ischemia-reperfusion." J Thorac Cardiovasc Surg. 114. 635-642 (1997)

Nakamura T、Hirata T、Fukuse T 等人：“二丁酰 c AMP 可减轻冷藏和缺血再灌注引起的肺损伤。”

Fukuse T, Hirata T, Wada H et al: "Comparison of saccharides on hypothermic pulmonary preservation : is efficacy related to the molecular weight?" Tarnsplantation Proceeding. 113(in press). (1998)

Fukuse T、Hirata T、Wada H 等人：“糖类对低温肺保存的比较：功效与分子量有关吗？”

Fukuse T,Hirata T,Wada H: "Effects of EC,UW,and new ET-Kyoto solutions in an ex vivo rat lung preservation model" Transplantation. 62. 1212-1217 (1996)

Fukuse T、Hirata T、Wada H：“EC、UW 和新 ET-Kyoto 解决方案在离体大鼠肺保存模型中的效果”移植。

Nakamura T,Hirata T,Fukuse T,et al.: "Dibutyryl c AMP attenuates lung injury caused by cold preservation and ischemia-reperfusion." J Thorac Cardiovasc Surg. 114. 635-642 (1997)

Nakamura T、Hirata T、Fukuse T 等人：“二丁酰 c AMP 可减轻冷藏和缺血再灌注引起的肺损伤。”

Ueda M, Wada H, et al: "Experimental tracheal transplantation for possible clinical application" Transplantation Proceeding. 29. 871-873 (1997)

Ueda M、Wada H 等人：“实验性气管移植以实现可能的临床应用”移植论文集。