CELL ADHESION AND LUNG PRESERVATION

采用大鼠体外再灌注模型，研究新型ET-K液在肺保存中的作用，并与EC液和UW液进行比较。灌流回路由30毫升同种异体血液启动。通过双头滚柱泵，血液从静脉储血器流经肺动脉，在被检查的肺中灌流。肺流出物以相同的流量回流到除氧器的新鲜肺。新鲜组用生理盐水灌洗肺，即刻复灌。新ET-K组、UW组和EC组分别用新ET-K组、UW组和EC组灌洗肺。新ET-K组和UW组的分流分数、肺动脉压和吸气峰压明显好于EC组，但与Fresh组基本相同。此外，该体外大鼠再灌注模型简单可靠，可广泛应用于肺保护的研究。我们观察了db-cAMP对冷保存和缺血再灌注所致肺损伤的保护作用。新鲜组用保存液冲洗肺，即刻进行再灌流。对照组和db-cAMP组分别用该液或db-cAMP液+db-cAMP液灌洗肺组织，保存于4ﾟC保存15h。Db-cAMP组再灌流肺的分流率与新鲜组一样低，明显低于对照组(p&lt；0.01)。Db-cAMP组肺血管内皮细胞损伤较轻。抗大鼠粒细胞抗体免疫组织化学显示db-cAMP组粒细胞较少。我们的结论是db-cAMP可以减轻冷保存和缺血再灌注所致的肺损伤，至少部分是通过保护血管内皮细胞实现的。

We study the effects of new ET-K solution in lung preservation, and compare it with EC and UW solutions using an ex vivo rat reperfusion model. The perfusion circuit was initiated by 30 ml of homologous blood. By means of double head roller pump, the blood passed from the venous blood reservoir through the pulmonary artery to be perfused in the examined lung. The lung effluent was returned at the same flow rate to the deoxygenator fresh lung. In fresh group, lung was flushed with saline, and then it was reperfused immediately. In the new ET-K group, UW group and EC group, lung was flushed with the new ET-K,UW,and EC,respectively. The shunt fraction, pulmonary arterial pressure, and peak inspiratory pressure in the new ET-K and UW groups were significantly better than those in the EC group, but were almost equal to those in the fresh group. In addition, this ex vivo rat reperfusion model is simple and highly reliable, and can be widely used in the studies of pulmonary preservation.We examined the effect of db-cAMP against lung injury caused by cold preservation and ischemia-reperfusion. In the fresh group, the lung was flushed with the preservative solution and reperfusion was performed immediately. In the control group and the db-cAMP group, the lung was flushed with either the solution or with a combination of the solution plus db-cAMP,respectively, and preserved at 4ﾟC at 15 hours. The shunt ratios of the reperfused lung in the db-cAMP group were as low as those in the fresh group and significantly lower than those in the control group (p<0.01). Electron microscopic examination showed less damage in te pulmonary arterial endothelium in the db-cAMP group. Immunohistochemical study using anti-rat granulocyte antibody showed less granulocytes in the db-cAMP group. We conclude that db-cAMP attenuates the lung injury by cold preservation and ischemia-reperfuion, at least partly by protection of the vascular endothelium.