Repair of nerve injury wrapped by encapsulated Schwann cells

包裹雪旺细胞包裹神经损伤的修复

• 批准号: 08671579
• 负责人: SAITOH Youichi
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671579/
• 关键词: Schwann cell; polymer capsule

To facilitate the regeneration of crush-injured facial nerve, purified Schwann cells were placed around the crush-injured nerve.Mature Schwann cells were obtained from dorsal root ganglia of SD rats as described by Kim et al. The two peripheral branches of left facial nerve of each SD rat (10 weeks old) were exposed, and pinched with aneurysm clip (Sugita No.1) for 2 minutes. The evoked electromyograms were recorded by stimulating the proximal and distal portions of the exposed facial nerve (8mA constant current). The mean distance between the two stimulation points was 25mm, and nerve conduction velocity (NCV) was calculated before injury, just after injury, and 14 days after injury. Ten crush-injured portions were covered with collagen gel (0.3ml) containing Schwann cells (1.0*10<@D18@>D1/ml) (Schwann group), and the other 8 were covered with collagen gel only (control group). After electrophysiological studies, the rats were perfused with 4% paraformaldehyde, and the nerves were removed and embedded in paraffin. The 5 mum-thick slices were stained for HE and S-100 protein immunocytochemically. The baseline NCV was 47.6(]SY.+-。[)6.8m/s (mean(]SY.+-。[)SE), and it reduced remarkably just after injury (11.8(]SY.+-。[)2.7m/s). Fourteen days after injury, NCV of Schwann group (30.3(]SY.+-。[)8.6m/s) was significantly greater than that of control group (11.2(]SY.+-。[)3.6m/s). However, immunocytochemistry failed to reveal transplanted Schwann cells around crush-injured nerve.From these results, transplantation of Schwann cells around crush-injured facial nerve may facilitate the nerve regeneration in the early period after crush-injury. However, it was difficult to determine the substance and mechanism of facilitation of the repair of injured nerves.

为促进面神经挤压伤后的再生，将纯化的雪旺细胞置于挤压伤神经周围，采用Kim等方法从SD大鼠背根神经节获得成熟的雪旺细胞，暴露10周龄SD大鼠左侧面神经的两个外周支，用Sugita No.1夹闭2 min。通过刺激暴露的面神经的近端和远端部分（8 mA恒定电流）记录诱发肌电图。两个刺激点之间的平均距离为25 mm，分别在伤前、伤后即刻和伤后14天测量神经传导速度（NCV）。10个挤压伤区用含雪旺细胞（1.0 × 10<@D18@>D1/ml）的胶原凝胶（0.3ml）覆盖（雪旺组），另8个挤压伤区仅用胶原凝胶覆盖（对照组）。电生理学研究后，大鼠用4%多聚甲醛灌注，取出神经并包埋在石蜡中。取5 μ m厚切片行HE和S-100蛋白免疫细胞化学染色。基线NCV为47.6（±）SY。[）6.8m/s（平均值（）SY. ±. [)SE)伤后即刻显著降低（11.8 ± 0.001）。（2.7m/s）。伤后14天，雪旺氏神经传导速度（30.3 ± 0.01）ms，雪旺氏神经传导速度（30.3 ± 0.01）ms，雪旺氏神经传导速度（30.3 ± 0.01）ms。（8.6m/s）显著大于对照组（11.2SY. ±.）。（3.6m/s）。结果表明，在挤压伤神经周围移植雪旺细胞，可促进挤压伤后早期神经再生。然而，目前尚难确定促进受损神经修复的实质和机制。

项目成果

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