The expression of growth hormone and insulin-like growth factor I recepter mRNAs in brai]tumors and tumor proliferation

生长激素和胰岛素样生长因子I受体mRNA在脑肿瘤中的表达及肿瘤增殖

• 批准号: 08671611
• 负责人: KAWANO Nobuyuki
• 金额: $ 1.22万
• 依托单位: KITASATO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671611/
• 关键词: brain tumor; IGF-I-R; GH-R; RT-PCR; IHC; IGF-1-R; ISH

The expression of growth hormone(GH) and insulin-like growth factor I(IGF-I) receptor mRNAs in brain tumors, and tumor proliferation.The relationship between the expression of GH receptor (GH-R) and IGF-I receptor (IGF-I R)mRNAs, and tumor proliferation has not been elucidated in human brain tumors. We investigated the expression of these receptors in 22 cases of astrocytic tumor and 20 cases of meningioma using not only reverse transcription-PCR(RT-PCR) but also immunohistochemical technique. In 22 case of astrocytic tumor, 11 cases were low grade astrocytoma and the rest were high grade one. Using RT-PCR,GH-R mRNA was found in 95% of meningiomas and in 86.4% of astrocytic trumors. IGF-I-R mRNA was found in 80% of meningiomas and in 91.0% of astrocytic tumors. GH-R and IGF-I-R mRNA in astrocytic tumors were expressed not only in high grade astrocytomas but also in low grade astrocytomas. Statistically, there were no significant relationship between the expression of these mRNAs and histological grade. In immunohistochemical study using frozen tissue, the distribution of IGF-I-R was diffusely recognized in the cytoplasm of meningioma cells and astrocytic tumor cells. On the other hand, IGF-I-R could not be detected in the tumor cells using paraffin embedded tissue. These results demonstrated the existence of IGF-I-R and GH-R in human astrocytic tumors and meningiomas. These receptors may play a role in the tumor cell proliferation and growth, and it is possible that not only IGF-I-R but also GH itself are one of growth accelerating factors in astrocytic tumors and meningiomas.We are now investigating the distribution of GH-R and IGF-I-R mRNAs in brain tumors using in site hybridization technique.

脑肿瘤中生长激素（GH）和胰岛素样生长因子I（IGF-I）受体mRNA的表达与肿瘤增殖的关系在人脑肿瘤中GH受体（GH-R）和IGF-I受体（IGF-IR）mRNA的表达与肿瘤增殖的关系尚不清楚。应用逆转录-聚合酶链反应（RT-PCR）和免疫组化技术检测了22例星形细胞瘤和20例脑膜瘤中这些受体的表达。22例星形细胞瘤中，低级别星形细胞瘤11例，高级别星形细胞瘤11例。用RT-PCR检测GH-R mRNA在脑膜瘤和星形细胞瘤中的阳性率分别为95%和86.4%。80%的脑膜瘤和91.0%的星形细胞瘤中有IGF-I-R mRNA表达。星形细胞瘤中GH-R和IGF-I-R mRNA不仅在高级别星形细胞瘤中表达，而且在低级别星形细胞瘤中也有表达。统计学上，这些mRNA的表达与组织学分级之间无显著相关性。冰冻切片免疫组化显示，IGF-I-R在脑膜瘤细胞和星形细胞瘤细胞胞浆中呈弥漫性分布。另一方面，IGF-I-R在石蜡包埋的肿瘤细胞中不能检测到。提示人脑星形细胞瘤和脑膜瘤中存在IGF-I-R和GH-R。这些受体可能在肿瘤细胞的增殖和生长中起作用，在星形细胞瘤和脑膜瘤中，不仅IGF-Ⅰ-R可能是促生长因子之一，GH本身也可能是促生长因子之一。

项目成果

H.Oka: "Preoperative superselective embolization of skull-base meningiomas : indications and limitations" J Neuro-Oncol. (in press).

H.Oka：“颅底脑膜瘤的术前超选择性栓塞：适应症和局限性”J Neuro-Oncol。

N.Kawano: "Pathologic features of ependymoma : Its histological patterns and a review of literature" Neuropathology. (in press).

N.Kawano：“室管膜瘤的病理特征：其组织学模式和文献综述”神经病理学。

N.Kawano: "Cerebellar clear cell ependymoma mimicking hemangioblastoma : Its clinical and pathological features" Surgical Neurology. (in press).

N.Kawano：“模仿血管母细胞瘤的小脑透明细胞室管膜瘤：其临床和病理特征”《神经外科》。

M.Takano: "Dural chondroma with fat tissue." Acta Neurochirur. 139. 690-691 (1997)

M.Takano：“带有脂肪组织的硬脑膜软骨瘤。”

N.Kawano: "Cerebellar clear cell ependymoma mimicking hemangioblastoma:Its clinical and pathological features." Surgical neurology. (in press).

N.Kawano：“模仿血管母细胞瘤的小脑透明细胞室管膜瘤：其临床和病理特征。”