The role of calcitriol in the immune system

1.骨化三醇（1，25 D）在血液透析患者（HDP）中的作用。体外I，25 D处理19例HDP和9例正常人外周血单核细胞（PBM）后，HLA-DR（DR）明显减少，单核细胞的吞噬功能明显增强，PMA诱导的超氧化物产生（SOP）也明显增加。HDP组中性粒细胞吞噬功能显著高于NS组，HDP组吞噬功能下降幅度大于NS组，结果表明，I，25 D能显著降低HDS患者外周血淋巴细胞对PWM的丝裂原反应（MR），但对Con A的反应不显著，并能显著增强PMA诱导的SOP，中性粒细胞的吞噬作用和淋巴细胞对Con A和PWM的MR不受此治疗的显著影响。2.1，25 D在泌尿系恶性肿瘤中的作用。(1)A在前列腺癌细胞系PC-3、PPC- 1和ALVA-10 1中发现了1，25 D的剂量依赖性抗增殖作用，而在阿尔瓦-41中没有发现。HLA-ABC（ABC）在PC-3、阿尔瓦-41和ALVA-101中表达，但在PPC-1中不表达。这些表达被干扰素-γ（IFN-γ）增强或诱导。DR不表达，也不被IFN-γ诱导。ICAM-1在所有细胞中表达，并被IFN-γ轻微上调。1，25 D对ABC、DR和ICAM-1无明显影响。（2）1，25 D对人肾癌细胞株KPK-1和SN 12 C无明显的抗增殖作用，IFN-γ能促进ABC的表达。DR在这些细胞中不表达，并且仅在KPK-1细胞中被LFN-gamma诱导。1，25 D对ABC和DR均无明显影响。

1. Calcitriol(1,25D) roles in hemodialysis patients(HDP).In vitro I , 25D treatment markedly reduced HLA-DR(DR) and stimulated phagocytosis in peripheral blood monocytes(PBM) from both 19 HDP and 9 normal subjects(NS).DR by monocytes and the phorbol myristate acetate(PMA)-induced superoxide production(SOP) by neutrophils were significantly higher in HDP than in NS.The phagocytosis in HDP decreased significantly more than that in NS.Moreover, the mitogen responses(MR) by peripheral blood lymphocytes also decreased significantly against PWM and insignificantly against Con A.In vivo I , 25D treatment resulted in a marked decrease in DR by PBM in HDS.Moreover, the treatment significantly enhanced the PMA-induced SOP.However, the phagocytosis by neutrophils and the MR to Con A and PWM by lymphocytes were not significantly affected by this treatment.2.1,25D effect in urologic malignancies.(1)A dose-dependent anti-proliferative effect of 1,25D was found in prostate cancer cell lines, PC-3, PPC- 1 and ALVA-lOl and not in ALVA-41. HLA-ABC(ABC) was expressed in PC-3, ALVA-41 and ALVA-lOl, but not in PPC-1. These expressions were either enhanced or induced by interferon-gamma (IFN-gamma). DR was not expressed and also not induced by IFN-gamma. ICAM-l was expressed in all cells and slightly up-regulated by IFN-gamma. There were no detectable effects of 1,25D on ABC, DR and ICAM-1.(2)Any anti-proliferative effect of 1,25D was not found in human renal cancer cell lines, KPK-l and SNl2C.ABC was expressed and enhanced by IFN-gamma. DR was not expressed in these cells and induced by LFN-gamma only in KPK-1 cells. There were no detectable effects of 1,25D on both ABC and DR.