子宮内膜癌抑制遺伝子の細胞老化活性を指標としたクローニング

为了明确人类1号染色体(HC-1)上与子宫内膜癌发生有关的衰老(抑制子)基因座，我们引入了两种Q臂缺失的HC-1，一种(del1-Q)在q12-q31之间存在间隙缺失，另一种(GM1173)几乎完全缺失Q臂。Del-1Q具有感官诱导活性，而GM1173没有。这一结果表明，该基因座位于1q31至tel之间。几个亚染色体可转导片段(STF)定位于Q31的某些区域，以传递到HHUA细胞。一株含有Q31-41的短链霉菌具有这种活性。在STF转移决定的区域，用连续的STS标记对癌症标本进行杂合分析后发现，衰老基因位于标记D1S225和D1S459两侧的区域内，位于1q41-42上。

In order to define the senescence (suppressor) gene locus involved in human endometriaI carcinogenesis on human chromosome 1 (HC-1), we introduced two kinds of the HC-1 which has deletions on q arm, one (del1-q) has interstitial deletion between q12-q31 and the other (GM1173) has almost complete deletion of q arm. Del-1q showed senscence-inducing activity but GM1173 did not. This result indicated the gene locus is carried on 1q31 to tel. Several subcromosomal transfferable fragments (STFs) addressed to certain regions of q31 to tel into HHUA cells. A STF harboring q31-41 had the activity. Following LOH study analyzing the cancer specimens by contiguous STS markers in the region determined by the STFs transfer suggested that the senescence gene locates within the region flanked by marker D1S225 and D1S459, on 1q41-42.