滲出性中耳炎中耳貯留液における分子生物学的細菌検索

渗出性中耳炎中耳液分子生物学细菌搜索

• 批准号: 08671980
• 负责人: 木村 貴昭
• 金额: $ 1.28万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671980/
• 关键词: 滲出性中耳炎; 中耳貯留液; RT-PCR; PCR; H. influenzae; M. catarrhalis; A. otitis

滲出性中耳炎は、近年小児期における本疾患の増加が問題とされる代表的な耳鼻咽喉科疾患である。一般に中耳貯留液よりはHaemophilus influenzae、Moraxella catarrharis、Streptococcus pneumoniaeが分離検出されると報告されているが、これらの細菌が中耳貯留液より分離されることは少なく、本疾患における細菌検索は十分に行われているとはいえない。われわれはこれらの細菌にたいするPCRプローベを作成し、滲出性中耳炎患者中耳貯留液内の細菌検出に応用、細菌感染の滲出性中耳炎への関与を検討した。滲出性中耳炎中耳貯留液内のH.infouenzae、M.catarrharisの検索をPCR法にて行った。その結果、中耳貯留液中の67%にてH.influenzaeが、26%にM.catarrharisが検出された。この検出率は、従来の細菌培養による検出率に比較してはるかに高いものであった。さらにPCR法による細菌検出と中耳貯留液の肉眼的症状につき比較検討したところ、mucoid貯留液においてserous貯留液に比較して有為に高くこれらの細菌が検出された。臨床経過との比較検討では、頻回の鼓膜切開を要した難治性例においてPCR法にてH.influenzae、M.catarrharisが高率に検出された。現在、滲出性中耳炎貯留液を経時的に採取し、中耳貯留液内細菌の検出を経時的に行っている。また近年薬剤耐性化が問題とされるS.pneumoniaeに関して、ペニシリン結合蛋白遺伝子の検出に基づく耐性化の検索をPCR法にて行っている。本法により、急性中耳炎患児鼻咽腔より分離されたS.pneumoniaeの約40%に薬剤耐性菌が検出された。

"Otorhinolaryngitis media", "Otorhinolaryngology disease" represented by "Otorhinolaryngology disease", "Otorhinolaryngology disease". Generally speaking, the retention of fluid in the middle ear is separated by Haemophilus influenzae, Moraxella catarrharis, and Streptococcus pneumoniae. The report reports that the fluid in the middle ear is separated, and the disease is caused by bacteria. The bacteria were isolated from the middle ear fluid of the patients with otitis media, and the bacteria were isolated from the middle ear fluid of the patients with otitis media. The bacteria were isolated from the middle ear fluid of the patients with otitis media, and the bacteria were infected. H.infouenzae and M.catarrharis were retained in the middle ear fluid of patients with otitis media. PCR method was used. The results showed that 67% of the middle ear fluid had H.influenzae, and 26% of the middle ear fluid had M.catarrharis. The rate of bacterial culture is higher than that of bacterial culture. The PCR method was used to detect the symptoms of the middle ear retention fluid, the naked eye symptoms, the mucoid retention fluid, the serous retention fluid, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the naked eye symptom, the middle ear retention fluid, the middle ear retention fluid, the middle ear In the bed, the tympanotomy and the myringotomy were used to treat the disease. The PCR method was used to treat the disease. The H.influenzae and M.catarrharis methods were highly effective. At present, when the retention fluid of otitis media is removed, and the bacteria in the retention fluid of the middle ear is removed, the bacteria in the retention fluid of the middle ear are removed. In recent years, the problem of S.pneumoniae tolerance is very important. In recent years, the problem of tolerance is very important. In recent years, the problem of tolerance is very important. In recent years, the problem of tolerance in recent years has been studied in this paper. The nasopharyngeal cavity was isolated from patients with acute otitis media. About 40% of S.pneumoniae was isolated and resistant bacteria were isolated from patients with acute otitis media.