Immunohistochemical study on cellular proliferative marker and genetic alteration

细胞增殖标志物和遗传改变的免疫组织化学研究

• 批准号: 08672154
• 负责人: IWAI Masayuki
• 金额: $ 0.45万
• 依托单位: Toyama Medical and Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672154/
• 关键词: Invasive Cancer; Cellular proliferative marker; Proto-oncogene; Immunohistochemistry; p53; cyclin D1; p34cdc2; bcl-2; び慢性浸潤癌; p^<53>; Cyclin D1; p^<34>; び漫性浸潤癌; サイクリンD_1; p34; 細胞増殖; PCNA; NORs

A series of studies were performed on the relations of cellular proliferative markers of PCNA, NORs, the genetic expressions of p53, Cyclin D1, p34cdc2, bcl-2 and apoptosis in tongue squamous cell carcinoma (SCC) with the mode of tumor invasion. The specimens from 67 tongue SCCs were used for this project. Immunohistochemical staining was employed to detect PCNA and the expression of genetic proteitns, and siverI staining method employed to examine NORs. The evaluation on those markers was performed by the positive rate of immunostained tumor cells calculated under microscopic field. The mode of tumor invasion was determoned by the method of Yamanoto and Kohama.Results1) PCNA was expressed with a lower positive rate (26.5*7.9) in tumors of mode 1-3, and with a high positive rate ( 41.1 * 6.7) in tumors of mode 4. 2) The numbers of NORs was found with a lower value (3.2*0.8) in tumors of mode 1-3, and with a relatively high value in tumors of mode 4.3) Cyclin Dl. was, with a relatively … More lower level, expressed in tumors with mode 1-3 (10/24, 41.3%), and with a high level in tumors with mode 4 (12/19, 92.3%).4) p53 expression was detected with a frequency in 16134 tumors with mode 1-3 (47.0%), and a relatively high frequecy in 9/13 tumors with mode 4 (69.2%).5) p34cdc2 expression was detected in 24/48 tumors with mode 1-3 (50.0%), and comparatively with a little high frequency in 10/15 tumors with mode 4 (62.7%).6) The apoptosis was labelled with the value of 2.53 *1.0 in tumors with mode 1-2, with the value of 1.95*1.5 in tumors with mode 3 and with the value of 1.5 * 1.04 in tumors with mode 4.7) bcl-2 expression was detected in 4/13 tumors with mode 1-2 (30.8%), in 11/27 tumors with mode 3 (40.07%) and in 9/11 tumors with mode 4 (81.8%).Conclusionln tongue SCC, the high level expressions of PCNA, NORs, Cyclin D1, p53, p34cdc2 and bcl-2 were more frequently detected in high invasive tumors. Conversely, apoptosis was labelled with relatively lower value in those tumors. These resuIlts suggest that the genetic abnormalities in relation to cellular proliferative activity involve in tumor invasion. Less

为探讨舌鳞状细胞癌(SCC)中增殖细胞核抗原(PCNA)、NORs、p53、Cyclin D1、p34cdc2、bcl2基因表达及细胞凋亡与肿瘤侵袭方式的关系。本研究共采集67例舌鳞癌标本。用免疫组织化学方法检测增殖细胞核抗原和基因蛋白的表达，用siverI法检测NORs。通过显微镜下计算免疫组织化学染色的肿瘤细胞阳性率来评价这些标记物。结果1)增殖细胞核抗原在模式1~3的肿瘤中阳性率较低(26.5±7.9)，在模式4的肿瘤中阳性率较高(41.1±6.7)。2)在模式1~3的肿瘤中，NORs的数目较低(3.2±0.8)，而在细胞周期蛋白Dl的肿瘤中表达较高。是，具有相对的…4)P53蛋白表达频率在模式1~3组16134例(47.0%)，模式4组9/13例(69.2%)。6)1~2期肿瘤细胞凋亡率为2.53×1.0，3期肿瘤细胞凋亡率为1.95×1.5，4.7期肿瘤细胞凋亡率为1.5×1.04。结论舌鳞状细胞癌中，高侵袭性肿瘤中，高表达的癌组织中，增殖细胞核抗原、NORs、Cyclin D1、P53、p34cdc2和bcl2的高表达更为常见。相反，在这些肿瘤中，细胞凋亡的标记值相对较低。这些结果提示，与细胞增殖活性相关的基因异常参与了肿瘤的侵袭。较少

项目成果

Itou S,Iwai M,et al: "Immunohistochemical Study of Cyclin D_1 produt in tongue SCC" Oral & Maxillofacial Surgery. 26・1. 176-176 (1997)

Itou S，Iwai M，等：“舌 SCC 中 Cyclin D_1 产品的免疫组织化学研究”口腔颌面外科 176-176。

岳麗華, 岩井正行 他: "舌癌における核小体形成体(AgNORs)の評価とその臨床的意義" 口腔腫瘍. 10・4. 244-253 (1998)

Reika Gaku、Masayuki Iwai 等：“舌癌中核仁形成物 (AgNOR) 的评估及其临床意义”口腔肿瘤 10・4 (1998)。

Itoh S, Iwai M, et al.: "Immunohistochemical study of cyclin D_1 product in tongue squamous cell carcinoma" Int.J.Oral and maxillofac.Surg.26・1. 176-176 (1997)

Itoh S、Iwai M 等人：“舌鳞状细胞癌中细胞周期蛋白 D_1 产物的免疫组织化学研究”Int.J.Oral and maxillofac.Surg.26・1（1997）。

Itou S,Iwai, M,et al.: "Immunohistochemical study of cyclin D1 product in tongue squamous cell carcinoma" Int.J.Oral and Maxillofac.Surg.V26.1. 176-176 (1997)

Itou S,Iwai, M,et al.：“舌鳞状细胞癌中细胞周期蛋白 D1 产物的免疫组织化学研究”Int.J.Oral 和 Maxillofac.Surg.V26.1。

Iwai M.Yao L.et al: "Apoptotic cell death in tongue squaorous cell Carcinoma with special reference to clinicopathological malignancy" Oral & Maxillofacial Surgery. 26・1. 266-266 (1997)

Iwai M.Yao L.等人：“舌鳞状细胞癌中的细胞凋亡，特别涉及临床病理学恶性肿瘤”26・1（1997）。