Regulation of osteogenic function of periodontal ligament fibroblast by 1d, 25-dihydroxy vitamin D3 receptor inducing factor.

1d，25-二羟基维生素D3受体诱导因子对牙周膜成纤维细胞成骨功能的调节。

• 批准号: 08672187
• 负责人: TAKIGAWA Masayuki
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672187/
• 关键词: periodontal ligament fibroblast; osteogenic function; 1alpha, 25-dihydroxy vitamin D3 receptor inducing factor; subtraction cDNA library; specific gene; サブトラクション cDNAライブラリー; サブトラクションcDNAライブラリー; 増殖因子

Regulation of osteogenic function of periodontal ligament fibroblast by 1alpha, 25-dihydroxyvitamin D3 receptor inducing factorCellular function of periodontal ligament fibroblasts (PLF) is regulated not only by exogenous factors such as hormones and cytokines, but also by endogenous factors derived from PLF.We have previously demonstrated that multilayred PLF produce a factor (s) which can induce the expression of 1alpha, 25-dihydroxyvitamin D3 receptor (VDR) in an autocrine manner. In this study, we first examined whether known growth factors could also induce the expression of VDR in PLF.The expression of VDR mRNA was induced by IGF-I,-II and EGF,but not by TGF-beta. This indicated that the expression of VDR in PLF could be regulated by several autocrine factors including these growth factors. We, therefore, tried to identify a gene (s) which is specific for PLF,and aimed to study the functions of PLF from a view point of the charactaristics of specific gene. Using confluent PLF and gingival fibroblasts (GF), we constructed a subtraction cDNA library which possibly contained several specific genes for PLF.34 clones were identified by Southern hybridization ; 5 were unknown genes and the other were highly homologous with a part of some known genes. Among the known genes, nm 23 protein or v-fos transformation effector protein, which were related to cell differentiation and proliferation, were included. These specific genes we obtained also might be related to some other important functions of PLF besides cell differentiation or osteogenic function, because they were obtained in comparison with GF from a same individual. It is necessary to classify these genes in the functions of PLF,and to examine whether the expression pattern of the specific gene is different among the differentiation stages of PLF.

1 α，25-二羟维生素D_3受体诱导因子对牙周膜成纤维细胞成骨功能的调控牙周膜成纤维细胞（PLF）的细胞功能不仅受外源性因子如激素和细胞因子的调节，而且还受PLF衍生的内源性因子的调节。25-二羟维生素D3受体（VDR）以自分泌方式。在本研究中，我们首先检测了已知的生长因子是否也能诱导PLF中VDR的表达，VDR mRNA的表达被IGF-I、IGF-II和EGF诱导，而不被TGF-β诱导。这表明VDR在PLF中的表达可能受到包括这些生长因子在内的多种自分泌因子的调节。因此，我们试图鉴定出PLF的特异性基因，并从该特异性基因的特性的角度来研究PLF的功能。利用融合的PLF和牙龈成纤维细胞（GF）构建了一个可能含有PLF特异性基因的消减cDNA文库，经Southern杂交鉴定出34个克隆，其中5个为未知基因，另1个与部分已知基因高度同源。在已知的基因中，包括与细胞分化和增殖相关的nm 23蛋白或v-fos转化效应蛋白。这些特异性基因的获得可能与PLF的细胞分化或成骨功能以外的其他重要功能有关，因为它们是与同一个体的GF比较获得的。有必要将这些基因在PLF的功能中进行分类，并检查特定基因的表达模式在PLF的分化阶段之间是否不同。

项目成果

Hideo Arai, Yoshio Nomura, Masahiko Kinoshita, Fusanori Nishimura, Masayuki Takigawa, Keiso Takahashi, Norifumi Washio, Shogo Takashiba, Yoji Murayama: "The inhibition of DNA synthesis by prostagland in E_2 in human gingival fibroblasts is independent of

Hideo Arai、Yoshio Nomura、Masahiko Kinoshita、Fusanori Nishimura、Masayuki Takikawa、Keiso Takahashi、Norifumi Washio、Shogo Takashiba、Yoji Murayama：“人牙龈成纤维细胞中 E_2 中前列腺素对 DNA 合成的抑制与

HH.Chou, S.Takashiba, M.Takigawa, F.Nishimura et al.: "Role of typeII interleukin-1 receptor expressed on human gingival tibroblast." Journal of Dental Research. 76 Special Issue. 406 (1997)

HH.Chou、S.Takashiba、M.Takikawa、F.Nishimura 等人：“人牙龈成纤维细胞上表达的 II 型白细胞介素 1 受体的作用”。

峯柴淳二,高柴正悟,大平泰資,大室博正,滝川雅之,西村英紀,村山洋二: "LPSが誘導する転写因子NF-KBを介したサイトカイン産生の抑制" 日本歯科保存学雑誌. 39巻 秋季特別号. 51- (1996)

Junji Mineshiba、Masago Takashiba、Yasushi Ohira、Hiromasa Omuro、Masayuki Takikawa、Hideki Nishimura、Yoji Murayama：“LPS 通过转录因子 NF-KB 诱导细胞因子产生的抑制”《日本保守牙科杂志》，秋季特刊。 51-（1996）

Yutaka Higuchi, Masayuki Takigawa, Hideo Arai, Norifumi Washio, Hyogo Ohe, Fusanori Nishimura, Hideki Shimizu, Yoshio Nomura, Shogo Takashiba, Yoji Murayama: "Effect of TNF-alpha on DNA synthesis and extracellular matrix protein synthesis in human gingiva

Yutaka Higuchi、Masayuki Takikawa、Hideo Arai、Norifumi Washio、Hyogo Ohe、Fusanori Nishimura、Hideki Shimizu、Yoshio Nomura、Shogo Takashiba、Yoji Murayama：“TNF-α 对人类牙龈 DNA 合成和细胞外基质蛋白合成的影响

Fusanori Nishimura, Victor P.Terranova, Braithwaite M,Rena Orman, Hideki Ohyama, Juniji Mineshiba, Hsin-Hua Chou, Shogo Takashiba, Yoji Murayama: "Comparison of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donor

Fusanori Nishimura, Victor P.Terranova, Braithwaite M,Rena Orman, Hideki Ohyama, Juniji Mineshiba, Hsin-Hua Chou, Shogo Takashiba, Yoji Murayama：“青少年和老年供体中人类牙周膜细胞体外增殖能力的比较