The Histological Study of Ridge-augmentation by Using Guided Tissue Regeneration

引导骨再生（Guided Bone Regeneration，GBR）是一种理想的骨缺损修复方法，但由于GBR所用材料的不可吸收性，存在膜暴露、感染和二次取出材料等问题，因此本研究旨在探讨骨缺损修复的可行性，方法：采用犬双侧下颌第一磨牙拔除3个月后，建立骨缺损动物模型。在无牙颌部分牙龈切开后，将颊侧黏骨膜瓣向舌侧提起，使骨外露，切除颊侧和舌侧5 mm × 5 mm的骨壁段，形成连续的颊舌侧骨缺损，实验组用棒状强化胶原膜覆盖骨缺损，以获得约5 mm高的间隙，对照组仅将牙龈瓣复位缝合。 关于我们 分别于术后即刻及术后12周用自制的牙垫、长锥技术及固定摄影条件拍摄X线片。在手术后/术后12周的X线片采用减影法进行评价分析，按一般规律制作近远中连续切片，染色后进行组织病理学检查。X线片观察，对照组对实验部位原始牙槽骨周径变化的认识较多，实验组对实验部位再生体积的认识较多，实验组对牙槽骨凹陷的认识较多实验组骨组织病理学观察显示，对照组骨再生较多，原始牙槽骨嵴向平移，新生骨与原始牙槽骨的边界部分较不明显，实验组骨组织中胶原膜残留较少，成膜部分周围未见炎性细胞。少

Guided Bone Regeneration (GBR) is demenstrated that is useful in ridge augmentation, but so that materials which is used with GBR is non-absorbable, those problems of infection with membrane exposure and the second operation of materials removal had been indicated.So the purpose of this study is to examine possibility of ridge augmentation in bone defects, with we develop absorbable membrane having take form, and using it in dog bone defect model.Bilateral mandibular first molars had been extracted 3 months previously and this study was started. After edentulous part gingiva was incised and buccal mucoperiosteal flaps were raised to ward the lingual side to expose bone, buccal and lingual bone wall segments measuring 5mm*5mm were removed to form bone defects, continuous bucco-lingually.The experimental group coated bone defects by rod reinforcement collagen membrane as can get space of approximately 5 mm high and the control group did only reposition suture of gingival flap.Periapical … More radiographs were obtained with individually manufactered bite blocks and the long-cone technique and fixed photography condition in right after operation and after an operation 12 weeks. There in right after operation/after an operation 12 weeks radiographs were evaluated analysis by Subtraction.We made mesiodistal serial sections according to universal law and there were stained for histopathological examination.The variate of circumference original alveoler bone of experiment parts was recognized much in control group and regeneration volume in experiment parts was recognized much in experimental group for radiographs.Concavity recognized alveoler bone much in control group and bone regeneration that original alveoler bone crest was shifted to flatly was recognized in experimental group for histopathological findings.boundary part of neogenesis bone and original alveoler bone was indistinctness relatively.The remain of collagen membrane was hardly recognized and the inflammatory cells wasn't observed in circumference of membrane setting part in experimental group. Less