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Anti-Liver Arginase Autoantibody in Human Serum : A New Tool for Diagnosis of Silent Chronic Liver Disease

人血清中的抗肝精氨酸酶自身抗体：诊断无症状慢性肝病的新工具

基本信息

批准号：
08672632
负责人：
MAFUNE Naoki
金额：
$ 0.64万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672632/
关键词：
arginase anti-liver arginase autoantibody chronic liver disease ELISA autoimmune disease 自己免疫疾患

项目摘要

Back ground/Aims : While there are a number of serum markers useful for the diagnosis of liver diseases, we meet some difficulty in diagnosing silent liver disorders such as chronic inactive hepatitis which does not show typical changes in releasing hepatic enzymes, symptomatic signs or deagnostic imaging. We have previously reported the induction of autoantibody to the liver arginase in animals by immunization with heterologous arginase and in human by allogeneic liver transplantation. The present study was initiated to utilize the anti-liver arginase autoantibody as a clinical marker in silent liver disorder. Methods : Western blot and ELISA were undertaken for the detection and quantification of the antiliver arginase autoantibody. In the ELISA system, rat liver arginase was used as a target antigen, taking advantage of its high immunological cross reactivity with human liver arginase and the ease of handling it provides. Results : ELISA for the anti-liver arginase autoantibody showed good reliability. Remarkable persistent increases in the anti-liver arginase autoantibody were found in chronic hepatitis and autoimmune hepatitis even in their inactive states. Conclusions : The anti-liver arginase autoantibody can be utilized as a clinical marker for diagnosing chronic inactive hepatitis.

背景/目标：虽然有许多血清标志物可用于诊断肝脏疾病，但我们在诊断无症状肝脏疾病如慢性非活动性肝炎时遇到了一些困难，慢性非活动性肝炎在释放肝酶、症状体征或诊断成像方面没有显示出典型的变化。我们之前曾报道过通过异源精氨酸酶免疫在动物中诱导针对肝脏精氨酸酶的自身抗体，以及通过异基因肝移植在人类中诱导针对肝脏精氨酸酶的自身抗体。本研究的目的是利用抗肝纤维化酶自身抗体作为无症状性肝病的临床标志物。方法：采用Western blot和ELISA方法检测和定量抗肝纤维化酶自身抗体。在ELISA系统中，利用大鼠肝过氧化物酶与人肝过氧化物酶的高免疫交叉反应性及其提供的易于处理性，将其用作靶抗原。结果：ELISA法检测抗肝纤维化酶自身抗体具有良好的可靠性。慢性肝炎和自身免疫性肝炎的抗肝纤维化酶自身抗体显著持续增加，即使在非活动状态下也是如此。结论：抗肝转移酶自身抗体可作为诊断慢性非活动性肝炎的一项临床指标。