Development of ultra-sensitive enzyme immunoassay for anti-acetylcholine receptor autoantibody

抗乙酰胆碱受体自身抗体超灵敏酶联免疫分析方法的研制

• 批准号: 08672656
• 负责人: KOHNO Takeyuki
• 金额: $ 1.41万
• 依托单位: Setsunan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672656/
• 关键词: Myasthenia Gravis; Acetylcholine receptor autoantibody; Enzyme immunoassay; Autoimmune Diseases; HLA; Epitope; 自己免症疾患

The pathobiochemical studies on myasthenia gravis (MG) by using a novel ultra-sensitive enzyme immunoassay is described. The results from this study is as follows.1. Ultra-sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using thirteen synthetic peptides, which corresponded to the sequence of the extra cellular domain of the acetylcholine receptor alpha-subunit, as antigens has been developed.2. The present method has enabled to definitively diagnose not only the generalized MG but also ocular MG (sensitivity=94%).3. In generalized MG patients, the present method has enabled to prognosticate the effectiveness of thymectomy before operation (sensitivity=64%, and specificity=90%) and the antibody titer has correlated with HLA genotype (DOB1^<**>0302, ^<**>0303 and ^<**>5031).4. In ocular MG patients, the class-II HLA genotype (DQB1^<**>03.) and class-I HLA (HLA-B) have correlated with effectiveness of the thymectomy.These results suggested that the pathogenicity of the generalized MG and ocular MG were related to humoral immune response and cellular immune response, respectively.

描述了使用新型超灵敏酶免疫分析法对重症肌无力 (MG) 进行的病理生化研究。本研究的结果如下： 1．开发了以与乙酰胆碱受体α亚基胞外结构域序列相对应的13种合成肽为抗原的超灵敏酶联免疫分析（免疫复合物转移酶联免疫分析）。 2．本方法不仅能够明确诊断全身性 MG，而且能够明确诊断眼部 MG（灵敏度=94%）。3．在全身性MG患者中，本方法能够在术前预测胸腺切除术的有效性（敏感性=64%，特异性=90%），并且抗体滴度与HLA基因型相关（DOB1^<**>0302、^<**>0303和^<**>5031）。 4．在眼部 MG 患者中，II 类 HLA 基因型（DQB1^<**>03.）和 I 类 HLA（HLA-B）与胸腺切除术的有效性相关。这些结果表明，全身性 MG 和眼部 MG 的致病性分别与体液免疫反应和细胞免疫反应有关。

项目成果

Masatoshi Hayashi et al: "Epitope analysisi of AChRAb in patients with myasthenia gravis using sensitive enzyme immunoassay." Ann. NY.Acad. Sci. (in press).

Masatoshi Hayashi 等人：“使用敏感酶免疫测定法对重症肌无力患者的 AChRAb 表位进行分析。”

Masatoshi Hayashi: "Epitope Analysis of AChRAb in Patients with Myasthenia Gravis Using Sensitive Enzyne Immunoassay" Ann.NY.Acad Sci. (in press).

Masatoshi Hayashi：“使用敏感酶免疫分析法对重症肌无力患者的 AChRAb 表位进行分析”Ann.NY.Acad Sci。