Novel functions of phospholipases

磷脂酶的新功能

• 批准号: 10212202
• 负责人: INOUE Keizo
• 金额: $ 62.59万
• 依托单位: Teikyo University (2000-2001)The University of Tokyo (1998-1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10212202/
• 关键词: platelet-activating factor; PHOSPHOLIPASE A; PHOSPHOLIPASE A1; PHOSPHOLIPASE A2; C.elegans; KNOCKOUT MICE; ホスホリパーゼA_1; ホスホリパーゼA_2; ノックアウトマウス; リゾホスファチジン酸; EDG7; ホスホリパーゼD; ホスファチジン酸; PAF; PAF-AH; 酸化リン脂質; リゾホスファチジルセリン

Physiological roles of three novel phospholipases have been analysed by producing knockout animals. The phospholipases are type I platelet-activating factor acetylhydrolase (PAF-AH (I)), type II platelet-activating factor acetylhydrolase (PAF-AH (II)), and phosphatidylserine-specific phospholipase A1 (PS-PLA1). For PAF-AH (I) and PS-PLA1, we have made knock out mice, and for PAF-AH (II), we have made knockout C.elegans, as the organism express mammalian homologue of PAF-AH (II). The phenotype of each animal are shown in table ;Table : Phenotype of knockout animalsanimals phenotypePAF-AH (I) mice abnormality on sperm formationPAF-AH (II) c.elegans abnormality in epidermis formationPS-PLA1 mice abnormality in T cell developmentAs these phenotype are not expected, novel function for each phospholipase is suggested, which should be solved in further studies.

通过产生基因敲除动物，分析了三种新型磷脂酶的生理作用。磷脂酶是 I 型血小板激活因子乙酰水解酶 (PAF-AH (I))、II 型血小板激活因子乙酰水解酶 (PAF-AH (II)) 和磷脂酰丝氨酸特异性磷脂酶 A1 (PS-PLA1)。对于PAF-AH (I)和PS-PLA1，我们制作了敲除小鼠，对于PAF-AH (II)，我们制作了敲除线虫，因为该生物体表达PAF-AH (II)的哺乳动物同源物。每只动物的表型如表所示;表：敲除动物的表型动物表型PAF-AH（I）小鼠精子形成异常PAF-AH（II）c.线虫表皮形成异常PS-PLA1小鼠T细胞发育异常由于这些表型是预期之外的，因此建议每种磷脂酶具有新的功能，应在进一步的研究中解决。

项目成果

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Nagai, Y. 等人：“磷脂酰丝氨酸特异性磷脂酶 A1 的另一种剪接形式，在人类中表现出溶血磷脂酰丝氨酸特异性溶血磷脂酶活性”J.

Nothwang,H.G. et al.: "Functional hemizygosity of PAFAH1B3 due to a PAFAH1B3-CLK2 fusion gene in a female with mental retardation. ataxia and atrophy of the brain"Hum.Mol.Gene.. (In press).

诺思旺，H.G.

K.Hayashi et al.: "Phenotypic Modulation of vascular smooth muscle cells induced by unsaturated lysophosphatidic acids"Circulation Res.. 89. 251-258 (2001)

K.Hayashi 等人：“不饱和溶血磷脂酸诱导的血管平滑肌细胞的表型调节”循环研究 89. 251-258 (2001)

Erickson J.R., et al.: "Lysophosphatidic acid and ovarian cancer : a paradigm for tumorogenesis and patient management"Prostaglandins Other Lipid Mediat. 64. 63-81 (2001)

Erickson J.R. 等人：“溶血磷脂酸和卵巢癌：肿瘤发生和患者管理的范例”前列腺素其他脂质介质。

Bandoh,K.et al.: "Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species-Structure-activity relationship of cloned LPA receptors-"FEBS letter. 478. 159-165 (2001)

Bandoh,K.等人：“EDG 家族的溶血磷脂酸 (LPA) 受体被 LPA 种类不同地激活 - 克隆 LPA 受体的结构-活性关系 -”FEBS 信。