Development of antibacterial agents targeting an extracellular signal transduction.

C的一根突变神经。perfringens strain 13，SI 112，它们产生了惊人的alpha-、kappa-和theta-toxins是孤立的当菌株SI 112与病毒/病毒突变菌株TS 133交叉接触时，从SI 112中生产的这些毒素在绵羊血琼脂板上明显可见。这表明，从TS 133中释放了一个提取物刺激物质，从SI 112激活了该物质的生产，但不能生产该物质。我们tentatively命名为VAP （virulence activating pheromone）和VAP。条件介质（CM）中的VAP主要在指数阶段产生，并在转录水平上激活了乙毒素生产。CM似乎对pfoA基因的刺激是依赖的。CM在添加后15分钟激活了pfoA转录，并指示VAP存在的快速响应。perfringens。在CM中的VAP似乎在37 ° C时30分钟的时间内极度不稳定地释放其活动，似乎是一个小分子（M.W.）（10 000人）由于C的病毒的这种提取物通信。perfringens对理解病理学和预防感染是重要的，我们现在正在尝试从斯特朗的文化介质中净化VAP。

One mutant strain of C. perfringens strain 13, SI112 that scarcely produce alpha-, kappa-, and theta-toxins was isolated. When the strain SI112 was cross-streaked with the virR/virS- mutant strain TS133, production of theta-toxin from SI112 was clearly observed in the crossing portion on a sheep blood agar plate. This indicated that an extracellular stimulating substance released from TS133 activated the production of theta-toxin from SI112 that could not produce the substance. We tentatively named the substance as VAP (virulence activating pheromone) and analyzed further. VAP in conditioned medium (CM) was produced mainly during the exponential phase and activated theta-toxin production at the transcriptional level. The stimulation of the pfoA gene by CM appeared to be dose-dependent. CM activated the pfoA transcription mostly at 15 min after addition, indicating a quick response to VAP exists in C. perfringens. VAP in CM seemed to be highly unstable loosing its activity within 30 min at 37゜C, and appeared to be a small molecule (M.W.<10,000). Since this extracellular communication for the virulence of C. perfringens would be important for understanding the pathogenicity and preventing infections, we are now trying to purify VAP from the culture medium of strain 13.