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Construction of periodontal ligament tissue around artificial dental roots

人工牙根周围牙周膜组织的构建

基本信息

批准号：
10557175
负责人：
OKUDA Reiichi
金额：
$ 6.72万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10557175/
关键词：
titanium periodontal ligament cells three-dimensional cell culture artificial dental root reconstruction of periodontal ligament periodontal epithelium epithelio-mesenchymal interaction newly formed cementum

项目摘要

1. The three-dimensional cell culture became possible of periodontal ligament cells with microcarrier. The adequate condition of the culture was clarified.2. Periodontal epithelial cells expressed alkaline phosphatase activity in vitro, not in vivo. It suggests that periodontal epithelial cells transformed in the culture environment.3. PDL cells controled osteoclastogenesis in vitro by express both ODF and OCIF mRNA.4. Decreasing of collagen αI(1) mRNA level and DNA methylation in the collagen αI(1) gene promotor region related to age-dependent degenerative alteration in periodontal tissue.5. Human periodontal ligament cells expressed CD14 mRNA and membrane CD14 at low level as compared with human gingival fibroblasts, and exhibited an only low response to LPS.6. It was suggested that periodontal epithelium (PE) had a role of the differentiation of cementoblasts during the acellular cementum formation. On the other hand, the PE in cellular cementum region was took part in the intermedi … More ate cementum and indicted the positive reaction of TUNEL.7. From the observation of healing process of the fenestration defect through the bone extended into the root surface, it was suggested that periodontal ligament could regenerate only from the original healthy periodontal ligament and the regeneration process of cementum was similar to the remodeling of bone.8. The cementum- and periodontal ligament-like tissue was observed on the titanium implants embedded in the cementum and periodontal ligament. On the other hand, bone was observed on the implants embedded in the alveolar bone.9. It was suggested that the cell-seeding of PDL cells into the intradentinal cavities enhanced the isolated cementum formation. The BrdU marked PDL cells were detected only in the seeded cavities by immunohistochemical method.10. The periodontal ligament-like tissue with newly formed cementum-like tissue was observed on the titanium embedded into the intradentinal cavities with cultured periodontal ligament cells in dogs.11. It is suggested that periodontal mechanosensitive neurons which were sensitive to the incisor and molar teeth project topographically to the different areas of the primary and secondary somatosensory cortex. Less

1. 微载体使牙周膜细胞的三维细胞培养成为可能。澄清了培养的适当条件。牙周上皮细胞在体外表达碱性磷酸酶活性，而在体内不表达。提示牙周上皮细胞在培养环境中发生了转化。PDL细胞通过表达ODF和OCIF mrna来控制体外破骨细胞的形成。牙周组织年龄依赖性退行性改变相关胶原αI(1)基因启动子区αI(1) mRNA水平下降及DNA甲基化。与人牙龈成纤维细胞相比，人牙周韧带细胞表达CD14 mRNA和膜CD14水平较低，且对lps的反应较低。结果表明，在脱细胞牙骨质形成过程中，牙周上皮对成牙骨质细胞的分化起一定作用。另一方面，细胞骨水泥区的PE参与了中间反应，更多地吞噬了骨水泥，并表现出tunel的正反应。从开窗缺损经骨延伸至根面愈合过程观察，提示牙周膜只能从原健康牙周膜再生，牙骨质的再生过程类似于骨的重塑。在牙骨质和牙周韧带内嵌钛种植体上观察到牙骨质和牙周韧带样组织。另一方面，在牙槽骨内嵌的种植体上观察到骨。结果表明，PDL细胞在牙釉质腔内的植入促进了离体牙骨质的形成。BrdU标记的PDL细胞仅在种子腔中被免疫组化检测到。用培养的牙周韧带细胞包埋在牙槽内后，观察到牙周韧带样组织与牙骨质样组织的形成。结果表明，对切牙和磨牙敏感的牙周机械敏感神经元分布在初级和次级体感皮层的不同区域。少