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Research of Chemiluminescence and Bioluminescence intend to create new functional System

化学发光和生物发光研究旨在创建新的功能系统

基本信息

批准号：
10640520
负责人：
KIMURA Masaru
金额：
$ 1.86万
依托单位：
KYUSHU UNIVERSITY (1999)Okayama University (1998)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1-1 : In order to prepare a high efficient chemiluminescent imidazole system, we tried to introduce an imidazole ring into the second position of rubrene which may help it to gain a very efficient chemiluminescent compound because of efficient fluorescence and low emission energy in an energy acceptor-emitter of the rubrene moiety.1-2 : Adiabatic photocycloreversion of biplanemers (4π+4π cycloadducts of aromatic compounds) is an excited state because the efficiency of the reaction can be monitored by the emission from the photoproduct (photo-chemiluminescence, PCL), and factors controlling the efficiency for excited product formation and shapes of potential surface of an excited state have been discussed.1-3 : The chemiluminescence compound 2-methyl-6-(4-methoxyphenol)imidazo[1, 2-α]pyrazin-3(7H)-one (MCLA) was attached to cyclomaltooligosaccharides (cyclodextrins) through a single spacer by the formation of an amide bond. The properties of oxygen-induced chemiluminescence of the synthesized cyclodextrin-bound MCLA were investigated in an aqueous phosphate buffer, pH 8.0. The light-emitting efficiency was remarkably dependent on the kind of bound cyclodextrin, spacer length between the MCLA and cyclodextrin, and the binding site in cyclodextrin. The light-emitting efficiencies of cyclomaltooctaose (γ-cyclodextrin)-bound compounds were higher than those of cyclomaltohexaose- or cyclomaltoheptaose-bound compounds.2 : dl-Cypridina luciferin and its analogs were synthesized from 2-aminopyrazine by an eight-step method that included two regio-selective Pd-mediated cross couplings, and their chemi- and bioluminescent actives, were compared.3 : We have scattered about 100 thiourea derivatives in order to develop a sensitive chemiluminescence detection for luminol derivatives. The proposed novel chemiluminescence reaction for luminol and luminol derivatives was applied to a high performance liquid chromatography (HPLC) assay for amino compounds.

1-1:为了制备高效的荧光咪唑体系，我们尝试在红荧烯的第二位引入咪唑环，这可能有助于其获得非常高效的荧光化合物，因为红荧烯部分的能量受体-发射体中的高效荧光和低发射能量。1 -2：双平面分子的绝热光旋回复（芳香族化合物的4π+4π环加合物）是激发态，因为反应的效率可以通过光产物的发射来监测（光致化学发光，PCL），以及控制激发产物形成的效率和激发态的势面形状的因素。1 -3：化学发光化合物2-甲基-6-（4-甲氧基苯酚）咪唑并[1，2-α]吡嗪-3（7 H）-酮（MCLA）通过酰胺键的形成通过单个间隔基连接到环麦芽糖寡糖（环糊精）上。在pH 8.0的磷酸盐缓冲溶液中，研究了合成的环糊精结合的MCLA的氧诱导化学发光的性质。环糊精的种类、环糊精与MCLA之间的间隔基长度以及环糊精的结合位置对MCLA的发光效率有显著影响。环麦芽八糖的发光效率（γ-环糊精）结合的化合物的荧光强度高于环麦芽六糖或环麦芽七糖结合的化合物。以2-氨基吡嗪为起始原料，通过两步区域选择性钯介导的交叉偶联反应合成了2：dl-Cypridina sparin及其类似物，并比较了它们的化学和生物发光活性。我们分散了大约100个硫脲衍生物，以发展一个灵敏的化学发光检测鲁米诺衍生物。将鲁米诺及其衍生物的化学发光反应应用于氨基化合物的高效液相色谱分析。