Polygyny in the ant Amblyopone silvestrii

本研究的目的是建立一种利用随机引物进行PCR扩增的DNA指纹图谱的方法，为进一步研究原始多雌制的西氏钝眼蚁的社会结构奠定基础。A. silvestrii是一种小型蚂蚁，其单个工蚁产生约400 - 700 ng的基因组DNA。以该DNA为模板，使用以下合成寡聚物作为PCR引物，所述PCR引物被设计用于微卫星基因座的锚3'或5'端：1）用于CAP-PCR的简并引物（3 '-锚定引物），即，(CA)7或（AC）7-DG或DA或DT或DC，和（GA）7或（AG）7-HG或HA或HT或HC。2)（GA）7ACTG、（GA）7ACTA、（GA）7ACTT、（GA）7ACTC、（AG）7CGTG、（AG）7CGTA、（AG）7CGTT和（AG）7CGTC。3)与前者相同的重复序列，但在3'端锚定有2个任意的核苷酸残基。4)6个重复的GA或GT锚定在5'端，具有3至5个任意核苷酸残基。5)无锚序列的重复序列如（CAG）5、（TAG）5、（加塔）4和（GCGT）4。西尔维斯特里。另一个同类引物也只在与RE消化结合时才检测到个体差异。虽然这些引物对该蚁亲属结构的分析，特别是以一夫多妻为中心的亲属结构的分析是有用的，但我们需要更多的引物。

The aim of this study is to establish the method of DNA flngerprin6g using PCR with arbitrary primers, which is then to be used for the future study on the social structure of the primitive, polygynous ant, Amblyopone sivlestrii. A. silvestrii is a small ant, whose single workers yielded c.400-700ng of genomic DNA. With this DNA as a template, the following synthetic oligomers were used as PCR primers designed to anchor 3' or 5' end of microsatellite loci: 1) Degenerate primers for CAP-PCR (3'-anchord primers), I.e., (CA)7 or (AC)7-DG or DA or DT or DC, and (GA)7 or (AG)7-HG or HA or HT or HC. 2) (GA)7ACTG, (GA)7ACTA, (GA)7ACTT, (GA)7ACTC, (AG)7CGTG, (AG)7CGTA, (AG)7CGTT, and (AG)7CGTC. 3) The same repeats as the former but anchored at 3' end with 2 arbitrary nucleotide residues. 4) 6 repeats of GA or GT anchored at 5' end with 3 to 5 arbitrary nucleotide residues. 5) Repeats without anchor sequence such as (CAG)5, (TAG)5, (GATA)4 and (GCGT)4.Among these primers, one primer of the above category 2 has successfully produced polymorphism between individuals of A. silvestrii. Another primer of the same category also detected individual difference only when combined with RE-digestion. Although these primers are useful for the analysis of kin-structure, especially centered on polygyny, of this ant, yet we need some further primers.