Functional dissection of motility initiation in sea-urchin sperm flagella

海胆精子鞭毛运动启动的功能解剖

• 批准号: 10640662
• 负责人: BABA Shoji A.
• 金额: $ 2.18万
• 依托单位: Ochanomizu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640662/
• 关键词: ciliary movement; flagellar movement; sea-urchin sperm; motility initiation; reactivation; dynein; image analysis; axoneme; ダイニン; グリシン; 屈曲波形; ディジタル画像解析

I improved the temporal and spacial resolution of stroboscopic micrography investing electronic circuitry for driving a high power xenon flash. This enabled us utilizing 35-mm microfilm of excellent granularity for higher spacial resolution. The new stroboscope could work at as high as 6 kHz. I wrote an image analysis software, Bohboh. exe, which can be executed on Microsoft Windows 9x/NT.The analysis of motility initiation in sea-urchin sperm flagella by these novel techniques revealed distinct motility states in stages: bend-unbend stage, p-bend-only stage and steady flagellar beating stage.The analysis of movement at relative high temporal resolution together with very high spacial resolution of sea-urchin sperm reactivated around 10 μM revealed clustered activation of motor molecule dynein local between one or two doublet pair and distributed helically along the axoneme.

我改进了频闪显微摄影的时间和空间分辨率，投资了驱动高功率氙气闪光灯的电子电路。这使我们能够利用35毫米的缩微胶片的优良粒度更高的空间分辨率。新的频闪仪可以在高达6 kHz的频率下工作。我写了一个图像分析软件，Bohboh。用这些新技术对海胆精子鞭毛运动起始的分析揭示了不同阶段的运动状态：弯曲-伸直阶段，在相对高的时间分辨率和极高的空间分辨率下，分析了海洋生物的运动，在10 μM左右激活的海胆精子中，运动分子动力蛋白呈聚集性激活，分布于一对或两对偶联体之间，并沿轴丝呈螺旋状沿着分布。

项目成果

Yoshihiro Mogami, Nozomi Tokunaga, and Shoji A. Baba: "longevity in space : Experiment of the life span of Paramecium cell clone in space"Adv. Space Res.. 23-12. 2087-2090 (1999)

最上义弘、德永希、马场庄司：“太空长寿：草履虫细胞克隆在太空中的寿命实验”Adv.

Momoko O. Miyake, Yoshihiro Mogami, Eri Kataoka, Eri Kajita, and Shoji A. Baba: "Improvement in time and space resolution of stroboscopic micrography using high power xenon flash"Rev. Sci. Instrum.. 69-1. 325-326 (1998)

Momoko O. Miyake、Yoshihiro Mogami、Eri Kataoka、Eri Kajita 和 Shoji A. Baba：“使用高功率氙闪光灯改善频闪显微摄影的时间和空间分辨率”Rev。

Yoshihiro Mogami: "Super-helix model:A physiological Model for gravitaxis of Paramecium"Adv. Space Res.. 21-8/9. 1291-1300 (1998)

最上义宏：“超螺旋模型：草履虫重力生理模型”Adv.

Yoshihiro Mogami: "Sugar-hehy model : A physiological model for gravitaxis of Paramecium"Adv. Space Res.. 21-8/9. 1291-1300 (1998)

最上义宏：“Sugar-hehy 模型：草履虫重力生理模型”Adv.

Yoshihiro Mogami, and Shoji A. Baba: "Super-helix model : A physiological model for gravitaxis of Paramecium"Adv. Space Res.. 21-8/9. 1291-1300 (1998)

Yoshihiro Mogami 和 Shoji A. Baba：“超螺旋模型：草履虫重力的生理模型”Adv.