Breeding of yellow flower Camellia from amphydiploid by using embryo culture

由于种植在黄花骆驼上的目的，我们已经审查了几次后续的实验：1。作为Camellia japonica和C的混合体Chrysantha是在增长的方式上诞生的，我们已经建立了一种用于变色龙的方法。Most morphological characters of F-D21-D2 hybrids （C. japonica x C. Chrysantha）从父母那里得到了中间，但有些人被观察到包括在C.日本或C.。Chrysantha。花的颜色的所有F型D21型混合动力车Were limitted from pale yellow to cream color，而混合动力车似乎是隐形的。作为一种通过Embryo文化培养出富有远见的Embryos治疗的结果，大量的斑点和不寻常的射击被发现。他们中的一些人似乎成了两栖动物。作为一个PCR-RFLP分析的结果，一个通过物种或部分发现的扩增区域的氯铂DNA、小电泳带模式。这些结果对骆驼属的分类是有用的。通过对越南收集的物种的审查，我们确定了三种新的物种，并将它们公布于众。

For the purpose of breeding on the yellow flower Camellia, we have examined several experiments as follows :1. As the hybrid embryos of Camellia japonica and C. chrysantha have aborted on the way of growth, we have established the method of embryo culture for genus Camellia.2. Most morphological characters of FィイD21ィエD2 hybrids (C. japonica x C. chrysantha) were intermediate from parents but some characters were observed to incline to C. japonica or C. chrysantha. Flower color of all FィイD21ィエD2 hybrids were limitted from pale yellow to cream color, and the hybrids have seemed to be stelile.3. As a result of colchicine treatment to adventitious embryos induced by embryo culture, many stout and unusual shoots were obtained. Some of them have been seemed to be amphidiploid.4. As a result on PCR-RFLP analysis of amplified region of chloroplast DNA, peculiar electrophoresis band pattern caused by species or section were obtained. These results have been useful for classification of genus Camellia.5. By examination about specimens collected in Vietnam, we have determined three specimens as new species and have made public them.