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Breeding of yellow flower Camellia from amphydiploid by using embryo culture

双二倍体黄花茶的胚培养选育

基本信息

批准号：
10660021
负责人：
HAKODA Naotoshi
金额：
$ 2.24万
依托单位：
Tokyo University of Agriculture & Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660021/
关键词：
Camellia Intersecific Hybrid Embryo culture Amphidiploid Morphological character Hybrid test PCR

项目摘要

For the purpose of breeding on the yellow flower Camellia, we have examined several experiments as follows :1. As the hybrid embryos of Camellia japonica and C. chrysantha have aborted on the way of growth, we have established the method of embryo culture for genus Camellia.2. Most morphological characters of FィイD21ィエD2 hybrids (C. japonica x C. chrysantha) were intermediate from parents but some characters were observed to incline to C. japonica or C. chrysantha. Flower color of all FィイD21ィエD2 hybrids were limitted from pale yellow to cream color, and the hybrids have seemed to be stelile.3. As a result of colchicine treatment to adventitious embryos induced by embryo culture, many stout and unusual shoots were obtained. Some of them have been seemed to be amphidiploid.4. As a result on PCR-RFLP analysis of amplified region of chloroplast DNA, peculiar electrophoresis band pattern caused by species or section were obtained. These results have been useful for classification of genus Camellia.5. By examination about specimens collected in Vietnam, we have determined three specimens as new species and have made public them.

为了进行黄花山茶的育种研究，我们进行了以下几个试验：1。作为山茶花和山茶花的杂种胚。金花茶在生长过程中发生败育，建立了山茶属植物胚培养的方法.杂种F1代D21代D2代（C. japonica × C. chrysantha）介于双亲之间，但有些性状偏向于C. japonica或C.菊花F1代D21代F2代D2代杂种的花色从淡黄色到乳白色不等，杂种表现为星型.用秋水仙素处理由胚培养诱导的不定胚，获得了许多粗壮、异常的新梢。其中一些似乎是双二倍体。通过对叶绿体DNA扩增区的PCR-RFLP分析，得到了种或组特异的电泳带型。这些结果对山茶属植物的分类有一定的参考价值.通过对采自越南的标本进行鉴定，确定其中3个为新种，并将其公布于世。