Phylogeographic study on Fagus crenata populations by the analysis on organelle DNA

基于细胞器DNA分析的水青冈种群系统发育地理学研究

• 批准号: 10660145
• 负责人: TOMARU Nobuhiro
• 金额: $ 2.56万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660145/
• 关键词: Fagaceae; Fagus crenata; mitochondrial DNA; genetic structure; phylogeography; genetic diversity; restriction fragment polymorphism; 葉緑体DNA

Objectives of this study is to make a phylogeographic map of Fagus crenata populations spread the entire disticution by investigating the haplotypes of organelles (mitochondria and choloroplast) and to find the relationship between geographic distribution and lineages of the populations. The conclusion is as follows.(1) Mitochondrial DNA variation and phylogeographic structure in 17F. crenata populationsMitochondrial DNA variation in 17F. crenata populations distributed throughout the species' range. Eight district haplotypes were identified using the restriction fragment length polymorphisms. Haplotype diversity within the populations was found to be very low, but population differentiation to be much higher. The mDNA variation was strikingly different from alloyzyme variation. Gene flow for maternally inherited mtDNA should be restricted to seed dispersal while nuclear gene flow occurs by both seed and pollen dispersal. Therefore, the difference in the variation between mtDNA and all … More ozymes may be largely a result of the much higher rate of gene flow associated with pollen dispersal than with seed dispersal. The mtDNA variation displayed strong geographic structure, which may reflect the species' distribution in the last glacial maximum and subsequent colonization.(2) Phylogeographic structure of F. crenata populations in Mt. Hakusan and the regions around itMtDNA variation in F. crenata was studied in five subpopulations of Hakusan populations and 19 populations distributed from central to western Honshu. Three haplotypes were detected within Hakusana populations and genetic structure was evident. Six haplotypes were detected from the 19 populations and the distribution revealed indicated that different lineages migrated into Mt. Hakusan in the processes of colonization after the last glaciation.(3) A phylogeographic map of F. crenata populationsA phylogeographic map of F. crenata populations was made using the mtDNA variation. The phylogeographic distribution of mtDNA revealed the relationships between geographic distribution and lineages of the populations. Less

本研究的目的是通过对毛茛（Fagus crenata）居群线粒体和叶绿体单倍型的研究，绘制分布于整个区域的毛茛居群的地理分布图，并探讨地理分布与居群谱系的关系。结论如下。(1)17 F.线粒体DNA变异与地理结构crenata种群17个F. crenata种群分布在整个物种的范围。利用限制性片段长度多态性鉴定了8个区域单倍型。种群内的单倍型多样性被发现是非常低的，但种群分化要高得多。mDNA变异与等位酶变异有显著差异。母系遗传mtDNA的基因流动应限于种子传播，而核基因流动则通过种子和花粉传播发生。因此，线粒体DNA和所有细胞之间的差异 ...更多信息 与种子传播相比，与花粉传播相关的基因流的速率要高得多。mtDNA的变异具有较强的地理结构性，可能反映了该物种在末次盛冰期的分布和随后的定居。(2)系统地理结构分析表明，F. crenata populations in Mt. Hakusan及其周围区域的MtDNA变异在F.对分布于本州中部至西部的19个居群和5个居群的白山居群进行了研究。在白草居群中检测到3种单倍型，遗传结构明显。从19个居群中检测到6种单倍型，其分布表明不同的谱系迁移到了黄山。最后一次冰期后的殖民化过程中的白山。(3)一幅F. crenata种群，绘制了F. crenata群体的mtDNA变异。线粒体DNA的地理分布揭示了地理分布与种群谱系的关系。少

项目成果

Tomaru,N.,Takahashi,M.,Tsumura Y.,Takahashi,M.and Ohba,K.: "Intraspecific variation and phylogeographic patterns of Fagus crenata(Fagaceae)mitochondrial DNA"American Journal of Botany. 85・5. 629-636 (1998)

Tomaru, N.、Takahashi, M.、Tsumura Y.、Takahashi, M. 和 Ohba, K.：“山毛榉（壳斗科）线粒体 DNA 的种内变异和系统发育模式”《美国植物学杂志》85・5。 636 (1998)