VITELLOGENESIS AND THE REGULATION OF VITELLOGENIN GENE EXPRESSION IN THE SCALLOP.

扇贝卵黄发生和卵黄原蛋白基因表达的调控。

• 批准号: 10660171
• 负责人: OSADA Makoto
• 金额: $ 2.18万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660171/
• 关键词: Scallop; Ovary; Vitellogenin; cDNA; Estrogen; Central Nervous System; Oocyte Growth

(1) Hormonal regulation of yolk protein formation and producing site of vitellogeninThe increase in yolk protein and total RNA contents in the scallop ovary during oocyte growth and the promoting effect of estradiol-17β(E_2) and cerebral and pedal ganglion (CPG) extract on yolk protein formation in in vivo and in vitro experiment indicated that ovary should be a producing site of vitellogenin and E_2 and a factor in CPG which is heat stable, trypsin and chymotrypsin resist and less than 10,000 of molecular weight promotively regulate vitellogenesis. The founding of estrogen receptor (ER) like immuno-reactivity only in the young oocytes suggested the autosynthetic system in the scallop vitellogenesis and the possibility of the E_2-induced vitellogenesis promoted by CPG based on the ER induction.(2) Cloning of vitellogenin cDNA and regulation of vitellogenin gene expression.Cloned vitellogenin cDNA (clone No. cSYP-1, Accession No. AB055960) from cDNA library of scallop ovary was an incom … More plete length (1698 bp) with initiation codon and a actual vitellogenin mRNA was found to be 12-13 kb according to northern blot analysis. A homology and molecular phylogenic tree analysis of the amino acid sequence deduced from cDNA sequence strongly supported that the sequence was vitellogenin cDNA.The expression of vitellogenin mRNA increased during oocyte growth, but still kept the same level at spawning season when the ovary was full of many full grown oocyte. In vitro culture associated with CPG showed no influence on vitellogenin mRNA level, very unlike vitellogenesis.From these results, the following tentative conclusion is proposed Endogenous estrogen induces vitellogenin in the oocyte via ER autosynthetically and vitellogenin is modified and accumulated into the oocyte. A factor from CPG which promotes the E_2-induced vitellogenesis enhances the vitellogenin gene transcription and the translation at the same time. Full grown oocyte terminate translation of vitellogenin mRNA which appeared to be long half-life. Less

(1)雌激素对卵黄蛋白形成的调节及卵黄蛋白原的产生部位雌激素17β（E_2）和脑足神经节（CPG）提取物对卵母细胞生长过程中卵巢中卵黄蛋白和总RNA含量的增加及体内、外卵黄蛋白形成的促进作用表明卵巢可能是卵黄蛋白原和E_2的产生部位。2和CPG中的一个热稳定因子，胰蛋白酶和糜蛋白酶抵抗，分子量小于10，000的促进性调节卵黄发生。雌激素受体（ER）样免疫反应仅在幼卵中发现，提示扇贝卵黄发生中存在自合成系统，CPG可能通过ER诱导来促进E_2诱导的卵黄发生。(2)从扇贝卵巢cDNA文库中克隆了卵黄蛋白原cDNA（clone No. cSYP-1，Accession No.AB055960），并进行了克隆、测序和序列分析。 ...更多信息 经北方杂交分析，该基因全长1698 bp，含有起始密码子和一个实际的卵黄蛋白原mRNA，大小为12-13 kb。同源性分析和分子进化树分析表明，该序列为卵黄蛋白原cDNA，卵黄蛋白原mRNA在卵母细胞发育过程中表达量增加，但在产卵季节卵巢中充满大量成熟卵母细胞时，其表达量仍保持不变。在体外培养条件下，CPG对卵黄蛋白原mRNA水平无影响，与卵黄发生不同，提示内源性雌激素通过自身合成ER诱导卵母细胞中的卵黄蛋白原，并使卵黄蛋白原被修饰和积累到卵母细胞中。CPG中的一个促进E_2诱导的卵黄发生的因子同时促进卵黄蛋白原基因的转录和翻译。成熟卵母细胞终止卵黄蛋白原mRNA的翻译，其半衰期较长。少

项目成果

M.Osada et,al.: "Vitellogenesis in the scallop, Patinopecten yessoensis, controled by estrogen and central nervous system."General and Comparative Endocrinology. (発表予定).

M.Osada 等人：“虾夷扇贝卵黄发生受雌激素和中枢神经系统控制。”普通和比较内分泌学（待提交）。

Makoto Osada et al.: "Expression of the vitellogenin gene in the oocyte of the scallop, Patinopecten yessoensis."Developmental Biology. (submitted).

Makoto Osada 等人：“卵黄蛋白原基因在扇贝虾夷扇贝卵母细胞中的表达。”发育生物学。

M.Osada et,al.: "Expression of the vitellogenin gene in the oocyte of the scallop, Patinopecten yessoensis."Developmental Biology. (発表予定).

M.Osada 等人：“卵黄蛋白原基因在扇贝虾夷扇贝卵母细胞中的表达。”发育生物学（待出版）。

M.Osada et al.: "Vitellogenesis in the scallop, Patinopecten yessoensis, controled by estrogen and central nervous system."General and Comparative Endocrinology. (発表予定).

M.Osada 等人：“虾夷扇贝的卵黄发生，由雌激素和中枢神经系统控制。”普通和比较内分泌学（待出版）。

M.Osada et al.: "Expression of the vitellogenin gene in the oocyte of the scallop, Patinopecten yessoensis."Developmental Biology. (発表予定).

M.Osada 等人：“卵黄蛋白原基因在扇贝虾夷扇贝卵母细胞中的表达。”发育生物学（待出版）。