Physiogenetical studies on effect of high environmental temperature on Japanese quail (Coturnix japonica)

高温环境对日本鹌鹑（Coturnix japonica）影响的生理遗​​传学研究

• 批准号: 10660275
• 负责人: WATANABE Seiki
• 金额: $ 1.79万
• 依托单位: Tokyo University of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660275/
• 关键词: Japanese quail; High temperature condition; liver; enzyme activity; heat shock protein (HSP) 70; 熱ショックタンバク質(HSP)70

Results of this research ca be summarized as follows.1. Japanese quail has high heat tolerance very much in comparison with chicken. The critical temperature is an environment temperature about 40 degrees, and physiological changes to show next are observed with adult female. (1) At 38 degrees, bards maintain the laying, and a change is hardly observed in the ovary and the liver. (2) At 40 degrees, the stop of the laying, the exhaustion of the sugar and stagnation of the fat composition in the liver is observed. But a part of the individual resumes the laying. (3) At 42 degrees, the laying stops completely, and an ovary and a liver are daunted, and the exhaustion of the sugar progresses further, and fat composition stops completely in the liver. The collapses of the liver cells are observed also.2. HSP 70 of Japanese quail is composed by at least two loci : CJHSP70A and CJHSP70B.The characteristics of CJHSP70A are as follows. (l) This gene expresses it in a liver, a kidney and a testis … More . (2) The amino acid sequence of this gene corresponds to the J02579 of chicken HSP70 gene that has the heat shock response 98%. (3) The base substitutions that do not influence amino acid sequence inside the protein binding region (PBR) are observed and these mutation can be distinguished easily by the PCR-RFLPs due to the recognition sites of restriction enzymes : Cfo I and HindIII.At least four allelic genes : pQAR22, pQBR24, pQGF29 and pQYR26 exist. On the other hand, the characteristics of CJHSP70B are as follows. (4) This gene is at least expressed in the liver. (5) The amino acid sequence of this gene corresponds to the AJ004940 of chicken HSP70 gene that expressed at the non-stress 99%. (6) At least two allelic genes : pQLZ011 and pQLZ131 based on the mutation of the recognition site of the restriction enzyme : HindIII exist.When an opportunity preface is made clear like the physiology concerned with heat resistance of the domestic fowl, this knowledge is very important, and it can contribute to the productivity improvement of the domestic fowl under the high temperature environment. Less

本研究的结果可概括如下： 1．与鸡肉相比，鹌鹑的耐热性非常高。临界温度是40度左右的环境温度，用成年女性观察接下来表现出的生理变化。 (1)38度时，吟游诗人维持产蛋，卵巢和肝脏几乎观察不到变化。 (2)在40度时，观察到产蛋停止，糖耗尽，肝脏中脂肪成分停滞。但个体的一部分恢复了产蛋。 (3)在42度时，产蛋完全停止，卵巢和肝脏受到威胁，糖的消耗进一步进行，脂肪成分在肝脏中完全停止。还观察到肝细胞的塌陷。2．日本鹌鹑的HSP 70由至少两个基因座组成：CJHSP70A和CJHSP70B。CJHSP70A的特征如下。 (l) 该基因在肝脏、肾脏和睾丸中表达......更多。 (2)该基因的氨基酸序列对应于鸡HSP70基因的J02579，具有98％的热休克反应。 (3) 观察到不影响蛋白质结合区 (PBR) 内氨基酸序列的碱基取代，并且由于限制性内切酶 Cfo I 和 HindIII 的识别位点，这些突变可以通过 PCR-RFLP 轻松区分。 至少存在 4 个等位基因：pQAR22、pQBR24、pQGF29 和 pQYR26。另一方面，CJHSP70B的特点如下。 (4)该基因至少在肝脏中表达。 (5)该基因的氨基酸序列对应于非应激99％表达的鸡HSP70基因的AJ004940。 (6)基于限制酶HindIII识别位点的突变，存在至少两个等位基因：pQLZ011和pQLZ131。当像与家禽的耐热性有关的生理学一样明确机会序言时，这一知识非常重要，它可以有助于提高家禽在高温环境下的生产力。较少的

项目成果

渡邉誠喜 他4名: "Sequencing and Allelic Analysis of Heat Shock Protein 70 Gene(HSP70) in Japanese Quail (Coturnix Japonica)"Thai Journal of Agricultural Science. 31(2). 149-163 (1998)

Seiki Watanabe 等 4 人：“日本鹌鹑 (Coturnix Japonica) 热休克蛋白 70 基因 (HSP70) 的测序和等位基因分析”，《泰国农业科学杂志》31(2) (1998)。

Attaporn SRICHAROEN.Takashi SHIINA.Akitaka MUKOYAMA, Kei HANZAWA.Seiki WATANABE: "Sequencing and Allelic Analysis of Heat Shock Protein 70 Gene (HSP70) in Japanese Quail (Coturnix Japonica)."Thai Journal of Agricultural Science. Vol.31, No.2. 149-163 (199

Attaporn SRICHAROEN.Takashi SHIINA.Akitaka MUKOYAMA、Kei HANZAWA.Seiki WATANABE：“日本鹌鹑 (Coturnix Japonica) 热休克蛋白 70 基因 (HSP70) 的测序和等位基因分析。”泰国农业科学杂志。