Measurement of vasorelaxant substance derived from endothelium and innervating nerve by biocascade system

Substances associated with endothelium-dependent or neurogenic relaxations were examined in isolated arteries and corpus cavernosum。Endothelium-derived relaxing substance other than nitric oxide(NO)and prostacyclin，and nerve-derived NO were tried to be measured by a biocascade system.(1)In isolated monkey coronary arteries，vasopressin-induced relaxation is mediated by NO released from the endothelium via stimulation of V ei D21基因D2 receptors.(2)In isolated monkey lingual arteries，acelycholine(ACh induced，endothelium dependent paritial)。The remaining relaxation was abolished by C I D12文件D1-dependent K I D1文件D1 channel blockers or cytochrome P450(CYP)3A-inhibitors.The biocascade system failed to detect the relaxant substance，possibly endothelium-dependent derived hyperpolarizing factor(EDHF)，indicating that the substance may be unstable。Products of arachidonic acid incubated with human liver microsome containing C…More YP3A relaxed the artery，the relaxation was abolished by the K I D1 I D 1 channel blockers.Products co-incubated with CYP3A-inhibitors did not relax the artery.These findings suggest that the relaxation by ACh in this artery is mediated by NO and K ei D1-channel opening substance(S)from arachidonic acid produced by CYP3A in the endothelium.(3)Histological studies with isolated canine corpus cavernosum demonstrated that saponin induced degenerative changes in the endothelial cells selectivity。ACh relaxed the intact，but not the saponin-treated，cavernos strips，the relaxation was partially inhibited by L-NA and the remaining relaxation was abolished by high concentration of K I D 1.On the other hand，neurogenic relaxation sensitive to L-NA was not affected by saponin.The nerve-derived NO was not detected by the biocascade system。These findings suggest that ACh acts on the endothelium and liberates NO and K I D1-channel opening substance which relax the smooth muscle of the cavernosum.Nitroxidergic nerve function appears to be unaffected by the damage of endothelial cells。Less：Less

Substances associated with endothelium-dependent or neurogenic relaxations were examined in isolated arteries and corpus cavernosum. Endothelium-derived relaxing substance other than nitric oxide (NO) and prostacyclin, and nerve-derived NO were tried to be measured by a biocascade system.(1) In isolated monkey coronary arteries, vasopressin-induced relaxation is mediated by NO released from the endothelium via stimulation of VィイD21ィエD2 receptors.(2) In isolated monkey lingual arteries, acelycholine (ACh)-induced, endothelium-dependent relaxation was partially inhibited by L-NA, a NO synthase inhibitor. The remaining relaxation was abolished by CィイD12+ィエD1-dependent KィイD1+ィエD1 channel blockers or cytochrome P450 (CYP) 3A-inhibitors. The biocascade system failed to detect the relaxant substance, possibly endothelium-dependent derived hyperpolarizing factor (EDHF), indicating that the substance may be unstable. Products of arachidonic acid incubated with human liver microsome containing C … More YP3A relaxed the artery, the relaxation was abolished by the KィイD1+ィエD1 channel blockers. Products co-incubated with CYP3A-inhibitors did not relax the artery. These findings suggest that the relaxation by ACh in this artery is mediated by NO and KィイD1+ィエD1-channel opening substance(s) from arachidonic acid produced by CYP3A in the endothelium.(3) Histological studies with isolated canine corpus cavernosum demonstrated that saponin induced degenerative changes in the endothelial cells selectivity. ACh relaxed the intact, but not the saponin-treated, cavernos strips, the relaxation was partially inhibited by L-NA and the remaining relaxation was abolished by high concentration of KィイD1+ィエD1. On the other hand, neurogenic relaxation sensitive to L-NA was not affected by saponin. The nerve-derived NO was not detected by the biocascade system. These findings suggest that ACh acts on the endothelium and liberates NO and KィイD1+ィエD1-channel opening substance which relax the smooth muscle of the cavernosum. Nitroxidergic nerve function appears to be unaffected by the damage of endothelial cells. Less